Breborowicz A, Karon J, Korybalska K, Martis L, Oreopoulos D G
Medical School Poznan, Poland.
Adv Perit Dial. 1995;11:15-8.
The authors tested in vitro the effect of glucose-based and amino acid-based dialysate effluent on the function of human peritoneal mesothelial cells. After 9 days of exposure to the tested effluents with medium (1:1 v/v) or to a medium supplemented with 10% fetal calf serum (FCS) (control), several functional properties of the cells were studied. The synthesis of DNA measured by incorporation of 3H-methyl-thymidine was higher in mesothelial-cell monolayers exposed to the dialysates than in the controls. Synthesis of hyaluronic acid was similar in all three groups, but after stimulation with Il-1 the cells exposed to the dialysates produced more hyaluronic acid. Synthesis of tissue plasminogen activator (t-PA) and plasminogen activator inhibitor-1 (PAI-1) was higher in the control cells. However, after stimulation with IL-1, the cells exposed to the dialysate showed greater synthesis of PAI-1 than of t-PA. Also, procoagulant activity of the control cells was higher than that of the cells exposed to the dialysates. We have concluded that the functional properties of the mesothelial cells may be altered in vitro during prolonged exposure to the dialysate, something that may also occur in vivo.
作者在体外测试了基于葡萄糖和基于氨基酸的透析液流出物对人腹膜间皮细胞功能的影响。在用培养基（1:1 v/v）或补充有10%胎牛血清（FCS）的培养基（对照）暴露于测试流出物9天后，研究了细胞的几种功能特性。通过掺入3H-甲基胸腺嘧啶核苷测量的DNA合成在暴露于透析液的间皮细胞单层中高于对照组。透明质酸的合成在所有三组中相似，但在用IL-1刺激后，暴露于透析液的细胞产生更多的透明质酸。组织纤溶酶原激活物（t-PA）和纤溶酶原激活物抑制剂-1（PAI-1）的合成在对照细胞中较高。然而，在用IL-1刺激后，暴露于透析液的细胞显示PAI-1的合成比t-PA的合成更多。此外，对照细胞的促凝血活性高于暴露于透析液的细胞。我们得出结论，在体外长时间暴露于透析液期间，间皮细胞的功能特性可能会改变，这在体内也可能发生。
