Fung E Y, Ewoldsen N O, St Germain H A, Marx D B, Miaw C L, Siew C, Chou H N, Gruninger S E, Meyer D M
Department of Oral Biology, University of Nebraska Medical Center, College of Dentistry, Lincoln 68583-0704, USA.
J Am Dent Assoc. 2000 Jan;131(1):51-8. doi: 10.14219/jada.archive.2000.0019.
Limited information is available regarding potentially estrogenic bisphenol A, or BPA, released from dental sealants. This study determined the rate- and time-course of BPA released from a dental sealant (Delton Opaque Light-cure Pit and Fissure Sealant, Preventive Care/Dentsply) when applied at a dosage of 8 milligrams (one tooth) or 32 mg (8 mg on each of four teeth) to 40 healthy adults.
The authors recruited 40 healthy subjects (18 men and 22 women, 20-55 years of age) who did not have histories of pit and fissure sealant placement or composite resin restorations. The authors collected saliva (30 milliliters) and blood (7 mL) specimens from all subjects immediately before sealant placement (baseline) and at one hour, three hours, one day, three days and five days after sealant placement. They used high-pressure liquid chromatography to determine BPA (detection sensitivity 5 parts per billion, or ppb) in all specimens.
The authors detected BPA in some saliva specimens (5.8-105.6 ppb) collected at one hour and three hours. The BPA, however, was not detectable beyond three hours or in any of the serum specimens. For the one- and three-hour saliva samples, the BPA concentration in the high-dose (32 mg) group was significantly greater than in the low-dose (8 mg) group (P < .05, Wilcoxon signed rank test). In the high-dose group, there was a significant decrease in saliva BPA concentrations from one hour to three hours (P < .01, Wilcoxon signed rank test).
This study showed that BPA released orally from a dental sealant may not be absorbed or may be present in nondetectable amounts in systemic circulation. The concern about potential estrogenicity of sealant may be unfounded.
关于牙科密封剂释放的潜在雌激素双酚A（BPA）的信息有限。本研究确定了一种牙科密封剂（Delton不透明光固化窝沟封闭剂，预防护理/登士柏）以8毫克（一颗牙）或32毫克（四颗牙各8毫克）的剂量应用于40名健康成年人时，BPA的释放速率和时间进程。
作者招募了40名健康受试者（18名男性和22名女性，年龄20 - 55岁），他们没有窝沟封闭剂放置或复合树脂修复史。作者在密封剂放置前（基线）以及放置后1小时、3小时、1天、3天和5天，从所有受试者收集唾液（30毫升）和血液（7毫升）样本。他们使用高压液相色谱法测定所有样本中的BPA（检测灵敏度为十亿分之五，即ppb）。
作者在1小时和3小时收集的一些唾液样本中检测到了BPA（5.8 - 105.6 ppb）。然而，3小时后或任何血清样本中均未检测到BPA。对于1小时和3小时的唾液样本，高剂量（32毫克）组的BPA浓度显著高于低剂量（8毫克）组（P < 0.05，Wilcoxon符号秩检验）。在高剂量组中，唾液BPA浓度从1小时到3小时有显著下降（P < 0.01，Wilcoxon符号秩检验）。
本研究表明，牙科密封剂经口释放的BPA可能不会被吸收，或者在体循环中的含量可能无法检测到。对密封剂潜在雌激素性的担忧可能是没有根据的。
