Mantani N, Andoh T, Kawamata H, Terasawa K, Ochiai H
Department of Japanese Oriental Medicine, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Sugitani.
Antiviral Res. 1999 Dec 31;44(3):193-200. doi: 10.1016/s0166-3542(99)00067-4.
Using several herbal extracts, we investigated whether certain Kampo medicines exert an inhibitory effect on the acidification of intracellular compartments such as endosomes and lysosomes (referred to as ELS), and thereby inhibit the growth of influenza A virus in Madin-Darby canine kidney cells. The vital fluorescence microscopic study showed that the extract of Ephedrae herba (EHext) among five herbal extracts inhibited acidification of endosomes and lysosomes in a concentration-dependent manner (100-400 microg/ml). Moreover the growth of influenza A/PR/8/34 (H1N1) (PR8) virus was inhibited when the cells were treated with EHext for 1 h immediately after infection, or treated as early as 5-10 min after infection. Conversely, virus growth resumed concomitantly with the reappearance of acidified ELS after removal of EHext. The fact that the inhibitory effect of EHext was completely or partially reversed by FeCl3, a tannin-reactive agent, strongly suggests that tannin is one of the active components in the extract.
我们使用了几种草药提取物，研究某些汉方药是否对细胞内区室（如内体和溶酶体，简称ELS）的酸化产生抑制作用，从而抑制甲型流感病毒在犬肾传代细胞中的生长。活体荧光显微镜研究表明，五种草药提取物中的麻黄草提取物（EHext）以浓度依赖方式（100 - 400微克/毫升）抑制内体和溶酶体的酸化。此外，当细胞在感染后立即用EHext处理1小时，或在感染后5 - 10分钟尽早处理时，甲型/PR/8/34（H1N1）（PR8）病毒的生长受到抑制。相反，去除EHext后，随着酸化的ELS重新出现，病毒生长随之恢复。单宁反应剂FeCl3能完全或部分逆转EHext的抑制作用，这一事实强烈表明单宁是该提取物中的活性成分之一。
