Helmy H, Weil G J, Faris R, Gad A M, Chandrashekar R, Ashour A, Ramzy R M
Research and Training Center on Vectors of Diseases, Ain Shams University, Cairo, Egypt.
Parasite Immunol. 2000 Feb;22(2):89-96. doi: 10.1046/j.1365-3024.2000.00280.x.
Human IgG antibody responses to Wuchereria bancrofti third stage infective larvae (L3) surface and somatic antigens were studied by indirect immunofluorescence (IFA) and immunoblot with endemic Egyptian sera (n = 115) with the aim of identifying targets of protective immunity. Human sera variably recognized 14 major bands in L3 by immunoblot. The statistical significance of group differences in antibody prevalence was assessed by the chi-squared test. Children and young adults (aged 10-20 years) tended to have antibodies to more L3 somatic antigens than older adults, with significant differences for bands at 66, 60 and 5 kDa. Infected subjects had more consistent antibody responses to antigens at 55, 50 and 6 kDa than endemic normal subjects with negative serum filarial antigen tests, who are presumed to be uninfected. A 5 kDa antigen was preferentially recognized by the latter group. Antibodies to L3 surface antigens were equally prevalent in uninfected children (75%) and adults (90%) but less prevalent in people with microfilaremia (38%) than in amicrofilaremic subjects with or without filarial antigenemia (81%) (P < 0.001). IFA-positive sera showed significantly enhanced recognition of antigens at 66, 40 and 14 kDa in immunoblots relative to IFA-negative sera. Additional studies are needed to further characterize antigens identified in this study and to establish whether they are indeed targets of protective immunity in humans.
通过间接免疫荧光法（IFA）和免疫印迹法，利用埃及流行区血清（n = 115）研究了人类对班氏吴策线虫第三期感染性幼虫（L3）表面和体抗原的IgG抗体反应，目的是确定保护性免疫的靶点。通过免疫印迹法，人类血清可不同程度地识别L3中的14条主要条带。采用卡方检验评估抗体流行率组间差异的统计学意义。儿童和年轻人（10 - 20岁）比对年龄较大的成年人倾向于拥有更多针对L3体抗原的抗体，在66 kDa、60 kDa和5 kDa条带处存在显著差异。与血清丝虫抗原检测呈阴性的流行区正常受试者（推测未感染）相比，感染受试者对55 kDa、50 kDa和6 kDa抗原的抗体反应更一致。后一组优先识别5 kDa抗原。未感染儿童（75%）和成年人（90%）中L3表面抗原抗体的流行率相当，但微丝蚴血症患者（38%）中的流行率低于有或无微丝蚴血症的无丝虫抗原血症受试者（81%）（P < 0.001）。相对于IFA阴性血清，IFA阳性血清在免疫印迹中对66 kDa、40 kDa和14 kDa抗原的识别显著增强。需要进一步研究以进一步表征本研究中鉴定的抗原，并确定它们是否确实是人类保护性免疫的靶点。
