牵张成骨中的生长因子。牵张成骨诱导的人骨痂中转化生长因子-β1和胰岛素样生长因子-I的免疫组织学模式。
Growth factors in distraction osteogenesis. Immuno-histological pattern of TGF-beta1 and IGF-I in human callus induced by distraction osteogenesis.
作者信息
Eingartner C, Coerper S, Fritz J, Gaissmaier C, Koveker G, Weise K
机构信息
BG Trauma Centre, University of Tubingen, Tübingen, Germany.
出版信息
Int Orthop. 1999;23(5):253-9. doi: 10.1007/s002640050365.
Although growth factors have been demonstrated during bone healing, their presence has not yet been confirmed in callus distraction. Therefore, in 3 patients we searched for cytokines during callus distraction. Bone biopsies were immuno-histochemically stained for TGF-beta1, IGF-I, TGF-beta type II receptor, IGF receptor, and proliferating cell nuclear antigen (PCNA). Histologically we found immature woven bone in the middle of the callus zone and increasing calcification and lamellar bone in the re-modelling zone. Osteoblasts and fibroblast-like cells in the middle zone, and osteoblasts in all zones stained for TGF-beta and its receptor. The number of positive staining cells related to proliferous activity as assessed both by PCNA, and by bone density in radiographs. IGF-I could be detected everywhere. In conclusion, growth factors are present in bone formation and in areas of re-modelling during callotasis. Their relation to proliferous activity and radiographic density supports their involvement in osteogenesis.
尽管在骨愈合过程中已证实生长因子的存在，但在骨痂牵张过程中其存在尚未得到证实。因此，我们对3例患者在骨痂牵张过程中寻找细胞因子。对骨活检标本进行免疫组织化学染色，检测转化生长因子-β1（TGF-β1）、胰岛素样生长因子-I（IGF-I）、TGF-βⅡ型受体、IGF受体和增殖细胞核抗原（PCNA）。组织学上，我们在骨痂区中部发现未成熟的编织骨，在重塑区钙化增加且有板层骨形成。中部区域的成骨细胞和成纤维样细胞以及所有区域的成骨细胞均对TGF-β及其受体呈阳性染色。通过PCNA以及X线片上的骨密度评估，阳性染色细胞数量与增殖活性相关。IGF-I在各处均可检测到。总之，在骨痂延长术的骨形成和重塑区域存在生长因子。它们与增殖活性和X线密度的关系支持它们参与骨生成。
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