Junkersdorf B, Bauer H, Gutzeit H O
Institut für Zoologie, Technische Universität Dresden, Dresden, Germany.
Bioelectromagnetics. 2000 Feb;21(2):100-6. doi: 10.1002/(sici)1521-186x(200002)21:2<100::aid-bem4>3.0.co;2-u.
We have studied the effect of extremely low frequency electromagnetic fields (ELF-EMF) in the presence of a second stressor (mild heat shock) on the expression of a lacZ reporter gene under the control of hsp16 or hsp70 promoters in two transgenic strains of C. elegans. The expression of the reporter gene was studied by scoring animals with induced beta-galactosidase activity after staining in toto or by biochemical quantitation of the enzyme activity, respectively. In our experimental setup we were able to expose the animals to 50 Hz magnetic flux density of 0-150 microT and at the same time control temperature with high precision (+/-0.1 degrees C). Experimental conditions were defined for which EMF strongly enhances the expression of the reporter gene.
我们研究了极低频电磁场（ELF - EMF）在存在第二种应激源（轻度热休克）的情况下，对两种秀丽隐杆线虫转基因品系中受hsp16或hsp70启动子控制的lacZ报告基因表达的影响。通过对整体染色后具有诱导型β - 半乳糖苷酶活性的动物进行评分，或分别通过对酶活性进行生化定量，来研究报告基因的表达。在我们的实验设置中，我们能够使动物暴露于0 - 150微特斯拉的50赫兹磁通密度下，同时以高精度（±0.1摄氏度）控制温度。确定了电磁场强烈增强报告基因表达的实验条件。
