枯草芽孢杆菌染色体中重复序列的稳定性
Stability of reiterated sequences in the Bacillus subtilis chromosome.
作者信息
Young M, Ehrlich S D
机构信息
Department of Biological Sciences, University College of Wales, Aberystwyth, Dyfed, United Kingdom.
出版信息
J Bacteriol. 1989 May;171(5):2653-6. doi: 10.1128/jb.171.5.2653-2656.1989.
Abstract
The instability of reiterated sequences in the Bacillus subtilis chromosome that was previously reported (M. Young, J. Gen. Microbiol. 130:1613-1621, 1984) results from the presence of a truncated pC194 replication origin together with an intact replication protein A gene in the amplified DNA. Removal of the truncated pC194 replication origin or inactivation of replication protein A stabilizes reiterated sequences, whereas provision of replication protein A in trans destabilizes them. We suggest that residual activity of protein A at the truncated replication origin generates single-stranded DNA, which stimulates recombination between repeated sequences and thus destabilizes amplified structures.
摘要
先前报道的枯草芽孢杆菌染色体中重复序列的不稳定性（M. 杨，《普通微生物学杂志》130:1613 - 1621，1984）是由于在扩增的DNA中存在截短的pC194复制起点以及完整的复制蛋白A基因。去除截短的pC194复制起点或使复制蛋白A失活可使重复序列稳定，而反式提供复制蛋白A则会使其不稳定。我们认为，蛋白A在截短的复制起点处的残余活性会产生单链DNA，这会刺激重复序列之间的重组，从而使扩增结构不稳定。
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