Conrad R, Klose M, Claus P
Max-Planck-Institut für terrestrische Mikrobiologie, D-35043 Marburg, Germany.
Appl Environ Microbiol. 2000 Feb;66(2):828-31. doi: 10.1128/AEM.66.2.828-831.2000.
The contribution of acetate- and H(2)/CO(2)-dependent methanogenesis to total CH(4) production was determined in excised washed rice roots by radiolabeling, methyl fluoride inhibition, and stable carbon isotope fractionation. Addition of > or = 20 mM phosphate inhibited methanogenesis, which then was exclusively from H(2)/CO(2). Otherwise, acetate contributed about 50 to 60% of the total methanogenesis, demonstrating that phosphate specifically inhibited acetotrophic methanogens on rice roots.
通过放射性标记、氟甲烷抑制和稳定碳同位素分馏法，测定了离体洗净水稻根中乙酸盐和H₂/CO₂依赖型产甲烷作用对总甲烷生成的贡献。添加≥20 mM的磷酸盐会抑制产甲烷作用，此时甲烷仅由H₂/CO₂产生。否则，乙酸盐对总产甲烷作用的贡献约为50%至60%，这表明磷酸盐特异性地抑制了水稻根上的乙酸营养型产甲烷菌。
