Cutrin J M, Olveira J G, Barja J L, Dopazo C P
Departamento de Microbioloxia e Parasitoloxia, Instituto de Acuicultura-Facultade de Bioloxia, Universidade de Santiago de Compostela, Santiago de Compostela 15706, Spain.
Appl Environ Microbiol. 2000 Feb;66(2):839-43. doi: 10.1128/AEM.66.2.839-843.2000.
A comparison was done of 231 strains of birnavirus isolated from fish, shellfish, and other reservoirs in a survey study that began in 1986 in Galicia (northwestern Spain). Reference strains from all of the infectious pancreatic necrosis virus serotypes were included in the comparison, which was done by neutralization tests and agarose and polyacrylamide gel electrophoresis of the viral genome. The neutralization tests with antisera against the West Buxton, Spajarup (Sp), and Abild (Ab) strains showed that most of the Galician isolates were European types Sp and Ab; however, many isolates (30%) could not be typed. Results from agarose gels did not provided information for grouping of the strains, since all were found to have genomic segments of similar sizes. Analysis of polyacrylamide gels, however, allowed six electropherogroups (EGs) to be differentiated on the basis of genome mobility and separation among segments, and a certain relationship between EGs and serotypes was observed. A wide diversity of electropherotypes was observed among the Galician isolates, and as neutralization tests showed, most of the isolates were included in EGs corresponding to European types Ab and Sp. Only 6.5% of the isolates had the electropherotype characteristic of American strains.
对1986年始于西班牙西北部加利西亚地区的一项调查研究中从鱼类、贝类及其他宿主分离出的231株双RNA病毒进行了比较。比较中纳入了所有传染性胰腺坏死病毒血清型的参考毒株，通过中和试验以及病毒基因组的琼脂糖和聚丙烯酰胺凝胶电泳进行。用针对韦斯特巴克斯顿、斯帕尤鲁普（Sp）和阿比尔德（Ab）毒株的抗血清进行的中和试验表明，大多数加利西亚分离株属于欧洲类型Sp和Ab；然而，许多分离株（30%）无法分型。琼脂糖凝胶的结果未提供菌株分组信息，因为所有分离株的基因组片段大小相似。然而，聚丙烯酰胺凝胶分析基于基因组迁移率和片段间的分离情况区分出了六个电泳组（EG），并且观察到EG与血清型之间存在一定关系。在加利西亚分离株中观察到了广泛的电泳型多样性，中和试验表明，大多数分离株属于与欧洲类型Ab和Sp相对应的EG。只有6.5%的分离株具有美国毒株的电泳型特征。
