Department of Fish Diseases, National Veterinary Research Institute, Pulawy, Poland.
J Fish Dis. 2020 Nov;43(11):1443-1451. doi: 10.1111/jfd.13249. Epub 2020 Aug 26.
Infectious pancreatic necrosis virus belongs to the genus Aquabirnavirus and family Birnaviridae. By VP2 gene similarity, aquatic birnavirus is clustered into seven genogroups. The aim of this study was to genetically analyse IPN viruses occurring on Polish fish farms.
Samples from freshwater fish mostly from 2012 to 2013 and from northern Poland were examined for the presence of IPN virus using isolation on cell cultures, real-time RT-PCR and RT-PCR. Fragments of 1,377 and 1,079 bp of the VP2 and VP5 genes, respectively, were sequenced, and the results were assembled into one consensus and analysed by Geneious software. The same VP2 gene region was compared and a phylogenetic tree generated by the neighbour-joining method and MEGA6 software.
All tested Polish isolates belonged to genogroup 5, like other European Spajurup isolates.
Our findings prove that there is only one IPN virus genogroup in Poland. Polish isolates show close relationships with each other. There is a close relationship between Polish isolates and isolates from Turkey, Spain and Iran. Isolate 57 is a separate branch related to isolates from the United States and Taiwan. This points to the likelihood of past virus introduction via import of stock from those countries.
传染性胰脏坏死病毒属于水生双RNA 病毒科双 RNA 病毒属。根据 VP2 基因的相似性，水生双 RNA 病毒被分为七个基因群。本研究旨在对波兰养殖场的 IPN 病毒进行基因分析。
对来自波兰北部淡水鱼类的样本进行了检测，这些样本主要采集于 2012 年至 2013 年期间，检测方法包括细胞培养物分离、实时 RT-PCR 和 RT-PCR。对 VP2 和 VP5 基因的 1377bp 和 1079bp 片段进行了测序，并使用 Geneious 软件将结果组装成一个共识序列进行分析。对相同的 VP2 基因区域进行了比较，并通过邻接法和 MEGA6 软件生成了系统发育树。
所有测试的波兰分离株均属于基因群 5，与其他欧洲 Spajurup 分离株相同。
我们的研究结果表明，波兰只有一个 IPN 病毒基因群。波兰分离株彼此之间关系密切。波兰分离株与来自土耳其、西班牙和伊朗的分离株密切相关。分离株 57 是一个单独的分支，与来自美国和中国台湾的分离株有关。这表明过去可能通过从这些国家进口鱼苗引入了病毒。
