通过核糖分型可区分引起菜豆细菌性褐斑病和晕疫病的丁香假单胞菌致病变种。
Pathovars of Pseudomonas syringae causing bacterial brown spot and halo blight in phaseolus vulgaris L. are distinguishable by ribotyping.
作者信息
González A J, Landeras E, Mendoza M C
机构信息
Servicio Regional de Investigación y Desarrollo Agrario, Consejería de Agricultura, Ganadería y Pesca, 33300-Villaviciosa, Spain.
出版信息
Appl Environ Microbiol. 2000 Feb;66(2):850-4. doi: 10.1128/AEM.66.2.850-854.2000.
Abstract
Ribotyping was evaluated as a method to differentiate between Pseudomonas syringae pv. phaseolicola and pv. syringae strains causing bacterial brown spot and halo blight diseases in Phaseolus vulgaris L. Ribotyping, with restriction enzymes BglI and SalI and using the Escherichia coli rrnB operon as the probe, differentiated 11 and 14 ribotypes, respectively, and a combination of data from both procedures yielded 19 combined ribotypes. Cluster analysis of the combined ribotypes differentiated the pathovars phaseolicola and syringae, as well as different clonal lineages within these pathovars. The potential of ribotyping to screen for correlations between lineages and factors such as geographical region and/or bean varieties is also reported.
摘要
对核糖体分型作为区分丁香假单胞菌菜豆致病变种和丁香致病变种菌株的一种方法进行了评估，这两种菌株会在菜豆上引发细菌性褐斑病和晕疫病。核糖体分型使用限制性内切酶BglI和SalI，并以大肠杆菌rrnB操纵子作为探针，分别区分出了11种和14种核糖体类型，两种方法的数据组合产生了19种组合核糖体类型。对组合核糖体类型进行聚类分析，区分出了菜豆致病变种和丁香致病变种，以及这些致病变种内不同的克隆谱系。还报告了核糖体分型在筛选谱系与地理区域和/或豆类品种等因素之间相关性方面的潜力。
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