Romantschuk M, Bamford D H
Department of Genetics, University of Helsinki, Finland.
Microb Pathog. 1986 Apr;1(2):139-48. doi: 10.1016/0882-4010(86)90016-1.
The phytopathogenic pseudomonad Pseudomonas syringae pv. phaseolicola causes halo blight of bean (Phaseolus vulgaris L.). Initiation of infection depends on the ability of the cells to adhere to the target cell surface. P. syringae pv. phaseolicola expresses pili, which are the receptors of the lipid-containing dsRNA bacteriophage phi 6. phi 6-resistant bacterial strains can be divided into different piliation types. It was possible to show that the adhesion of the bacteria onto plant cell surface was dependent on the pili. Non-piliated bacterial stains showed a much lower adherence to the leaf surface than strains expressing phi 6 specific pili. Scanning electron microscopy showed that the piliated bacteria attached to the leaf surface at the site of stomata. Non-piliated bacteria were evenly distributed on the leaf surface. All bacterial strains used in this study were capable of causing halo blight if injected into the plant. If the bacteria were sprayed on the plants, followed by spraying of sterile buffer, only piliated bacteria caused symptoms.
植物病原性假单胞菌丁香假单胞菌菜豆致病变种可引发菜豆（菜豆属普通菜豆）的晕疫病。感染的起始取决于细胞黏附到靶细胞表面的能力。丁香假单胞菌菜豆致病变种表达菌毛，菌毛是含脂质双链RNA噬菌体φ6的受体。对φ6具有抗性的细菌菌株可分为不同的菌毛类型。已证实细菌在植物细胞表面的黏附取决于菌毛。无菌毛的细菌菌株与表达φ6特异性菌毛的菌株相比，对叶片表面的黏附性要低得多。扫描电子显微镜显示，有菌毛的细菌在气孔部位附着于叶片表面。无菌毛的细菌均匀分布在叶片表面。本研究中使用的所有细菌菌株如果注入植物体内都能够引发晕疫病。如果将细菌喷洒在植物上，随后再喷洒无菌缓冲液，只有有菌毛的细菌会引发症状。
