Vidigal T H, Caldeira R L, Simpson A J, Carvalho O S
Centro de Pesquisas René Rachou, Fiocruz, Belo Horizonte, MG, 30190-002, Brasil.
Mem Inst Oswaldo Cruz. 2000 Jan-Feb;95(1):57-66. doi: 10.1590/s0074-02762000000100009.
The polymerase chain reaction and restriction fragment length polymorphism (RFLP) of the internal transcribed spacer (ITS) region of the rRNA gene, using the enzyme DdeI were used for the molecular identification of ten species and one subspecies of Brazilian Biomphalaria. Emphasis is given to the analysis of B. oligoza, B. schrammi and B. amazonica. The RFLP profiles obtained using this enzyme were highly distinctive for the majority of the species and exhibited low levels of intraspecific polymorphism among specimens from different regions of Brazil. However, B. peregrina and B. oligoza presented very similar profiles that complicated their identification at the molecular level and suggested a very close genetic similarity between the two species. Others enzymes including HaeIII, HpaII, AluI and MnlI were tested for their ability to differentiate these species. For B. amazonica three variant profiles produced with DdeI were observed. The study demonstrated that the ITS contains useful genetic markers for the identification of these snails
利用聚合酶链反应和rRNA基因内部转录间隔区（ITS）的限制性片段长度多态性（RFLP），使用DdeI酶对巴西双脐螺属的10个物种和1个亚种进行分子鉴定。重点分析了少脐双脐螺、施氏双脐螺和亚马逊双脐螺。使用该酶获得的RFLP图谱对大多数物种具有高度特异性，并且在来自巴西不同地区的标本中种内多态性水平较低。然而，奇异双脐螺和少脐双脐螺呈现出非常相似的图谱，这使得它们在分子水平上的鉴定变得复杂，并表明这两个物种之间存在非常密切的遗传相似性。还测试了其他酶，包括HaeIII、HpaII、AluI和MnlI区分这些物种的能力。对于亚马逊双脐螺，观察到用DdeI产生的三种变异图谱。该研究表明，ITS包含用于鉴定这些蜗牛的有用遗传标记。
