Caldeira R L, Vidigal T H, Matinella L, Simpson A J, Carvalho O S
Centro de Pesquisas René Rachou, Fundação do Instituto Oswaldo Cruz, Belo Horizonte, MG, 30190-002, Brasil.
Mem Inst Oswaldo Cruz. 2000 Mar-Apr;95(2):171-7. doi: 10.1590/s0074-02762000000200007.
Snails of the genus Biomphalaria from Venezuela were subjected to morphological assessment as well as polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis. Morphological identification was carried out by comparison of characters of the shell and the male and female reproductive apparatus. The PCR-RFLP involved amplification of the internal spacer region ITS1 and ITS2 of the RNA ribosomal gene and subsequent digestion of this fragment by the restriction enzymes DdeI, MnlI, HaeIII and MspI. The planorbids were compared with snails of the same species and others reported from Venezuela and present in Brazil, Cuba and Mexico. All the enzymes showed a specific profile for each species, that of DdeI being the clearest. The snails were identified as B. glabrata, B. prona and B. kuhniana.
对来自委内瑞拉的双脐螺属蜗牛进行了形态学评估以及聚合酶链反应和限制性片段长度多态性（PCR-RFLP）分析。通过比较贝壳以及雌雄生殖器官的特征进行形态学鉴定。PCR-RFLP包括对核糖体RNA基因的内部间隔区ITS1和ITS2进行扩增，随后用限制性内切酶DdeI、MnlI、HaeIII和MspI对该片段进行消化。将这些平卷螺与来自委内瑞拉以及巴西、古巴和墨西哥的同物种和其他报道的蜗牛进行比较。所有酶对每个物种都显示出特定的图谱，其中DdeI的图谱最清晰。这些蜗牛被鉴定为光滑双脐螺、前双脐螺和库氏双脐螺。
