Spatz L, Vidigal T H, Silva M C, Cappa S M, Carvalho O S
Departamento de Microbiología, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina.
Mem Inst Oswaldo Cruz. 2000 Nov-Dec;95(6):807-14. doi: 10.1590/s0074-02762000000600010.
The correct identification of Biomphalaria oligoza, B. orbignyi and B. peregrina species is difficult due to the morphological similarities among them. B. peregrina is widely distributed in South America and is considered a potential intermediate host of Schistosoma mansoni. We have reported the use of the polymerase chain reaction and restriction fragment length polymorphism analysis of the internal transcribed spacer region of the ribosomal DNA for the molecular identification of these snails. The snails were obtained from different localities of Argentina, Brazil and Uruguay. The restriction patterns obtained with MvaI enzyme presented the best profile to identify the three species. The profiles obtained with all enzymes were used to estimate genetic similarities among B. oligoza, B. peregrina and B. orbignyi. This is also the first report of B. orbignyi in Uruguay.
由于Biomphalaria oligoza、B. orbignyi和B. peregrina这几个物种之间形态相似，因此很难对它们进行正确鉴别。B. peregrina在南美洲广泛分布，被认为是曼氏血吸虫的潜在中间宿主。我们已经报道了利用核糖体DNA内部转录间隔区的聚合酶链反应和限制性片段长度多态性分析来对这些螺蛳进行分子鉴别。这些螺蛳取自阿根廷、巴西和乌拉圭的不同地点。用MvaI酶获得的限制性图谱呈现出鉴别这三个物种的最佳特征。用所有酶获得的图谱用于评估B. oligoza、B. peregrina和B. orbignyi之间的遗传相似性。这也是乌拉圭关于B. orbignyi的首次报道。
