Saito K, Maeda M, Yoshihara H, Amano K, Nishijima M, Nakamura K
Department of Obstetrics and Gynecology, Kitasato University School of Medicine, Kanagawa, Japan.
Biol Neonate. 2000 Feb;77(2):109-14. doi: 10.1159/000014204.
The influence of oxidative stress in rat brain and liver on intrauterine growth retardation (IUGR) in rat fetuses was examined. Twenty pregnant Wistar rats were used. On the 15th day of pregnancy, uterine artery and vein were ligated bilaterally using a modified Wigglesworth method. On the 21st day of pregnancy, the fetuses were delivered by hysterotomy. Fetal blood was collected by cardiac puncture. Fetal brain and liver were removed for the analysis of lipid peroxide. Sham surgical operations were performed in the control rats. Within the ligated group, a superoxide dismutase mimicking substance, Fe-chlorine e6-Na (FeCNa), was injected intraperitoneally once a day from day 15 of gestation to day 20. Fetal blood, brain, and liver were stored at -70 degrees C until analysis. Control rats received an equivalent volume of saline. In growth-retarded fetuses, both superoxide released from erythrocytes and brain lipid peroxide showed significantly higher levels, but not superoxide dismutase in erythrocytes and liver lipid peroxide. These changes were alleviated by injection of superoxide dismutase-mimicking substance, FeCNa. Rat fetuses with intrauterine growth retardation suffered from a significant oxidative stress in utero. The increase in reactive oxygen species was alleviated by an injection of FeCNa.
研究了大鼠脑和肝脏中的氧化应激对大鼠胎儿宫内生长迟缓（IUGR）的影响。使用了20只怀孕的Wistar大鼠。在妊娠第15天，采用改良的Wigglesworth方法双侧结扎子宫动脉和静脉。在妊娠第21天，通过子宫切开术娩出胎儿。通过心脏穿刺收集胎儿血液。取出胎儿的脑和肝脏用于分析脂质过氧化物。对对照大鼠进行假手术操作。在结扎组中，从妊娠第15天到第20天，每天腹腔注射一次超氧化物歧化酶模拟物Fe-氯e6-Na（FeCNa）。将胎儿血液、脑和肝脏储存在-70℃直至分析。对照大鼠接受等量的生理盐水。在生长迟缓的胎儿中，红细胞释放的超氧化物和脑脂质过氧化物水平均显著升高，但红细胞中的超氧化物歧化酶和肝脏脂质过氧化物水平未升高。注射超氧化物歧化酶模拟物FeCNa可减轻这些变化。患有宫内生长迟缓的大鼠胎儿在子宫内遭受明显的氧化应激。注射FeCNa可减轻活性氧的增加。
