Peluso M, Airoldi L, Magagnotti C, Fiorini L, Munnia A, Hautefeuille A, Malaveille C, Vineis P
Servizio di Oncologia Sperimentale, Istituto Nazionale per la Ricerca sul Cancro (IST), Largo R.Benzi 10, Genova 16132, Italy.
Carcinogenesis. 2000 Feb;21(2):183-7. doi: 10.1093/carcin/21.2.183.
The 'Mediterranean diet', a diet rich in cereals, fruit and vegetables, has been associated with lowering the risk of a variety of cancers of the digestive tract and the bladder. In a previous study, we showed that the high phenolic content these dietary components produce in the urine could be associated with higher antimutagenic properties of the urine and lower arylamine-DNA adducts in exfoliated bladder cells. We have conducted a case-control study on 162 bladder cancer patients and 104 hospital controls. Total aromatic DNA adducts were measured in white blood cells (WBC) of all subjects by (32)P-post-labelling. Genetically based metabolic polymorphisms were analysed by PCR-RFLP (NAT2, GSTM1, GSTT1, GSTP1, COMT and NQO1). All subjects were interviewed about their tobacco use, dietary habits and other risk factors. The odds ratio (OR) for the risk of bladder cancer according to the presence/absence of WBC DNA adducts (detection limit 0.1 RALx10(8)) was 3.7 [95% confidence interval (CI) 2.2-6.3] and a dose-response relationship with levels of adducts was apparent. The association between case/control status and the presence of WBC DNA adducts was significantly stronger in the subjects who consumed fewer portions of fruit or vegetables per day (OR 7.80, 95% CI 3.0-20.30 for 0-1 portions of vegetables) than in the heavy consumers (OR 4.98 for consumers of 2 portions daily, OR 1.97 for consumers of > or =3 portions; similar but lower estimates were found for the intake of fruit). No association was noticed between tobacco smoking and WBC DNA adducts. Only NAT-2, among the several genotypes considered, was associated in a statistically significant way with the risk of bladder cancer (OR 1.72, 95% CI 1.03-2.87) and with the levels of WBC DNA adducts. Our report suggests that fruit and vegetables could protect against bladder cancer by inhibiting the formation of DNA adducts.
“地中海饮食”富含谷物、水果和蔬菜，与降低多种消化道癌症和膀胱癌的风险有关。在之前的一项研究中，我们发现这些饮食成分在尿液中产生的高酚含量可能与尿液中更高的抗诱变特性以及脱落膀胱细胞中较低的芳胺 - DNA加合物有关。我们对162例膀胱癌患者和104例医院对照进行了一项病例对照研究。通过³²P后标记法测量所有受试者白细胞（WBC）中的总芳香族DNA加合物。通过PCR - RFLP（NAT2、GSTM1、GSTT1、GSTP1、COMT和NQO1）分析基于基因的代谢多态性。所有受试者都接受了关于其吸烟情况、饮食习惯和其他风险因素的访谈。根据白细胞DNA加合物的存在与否（检测限为0.1 RALx10⁸），膀胱癌风险的比值比（OR）为3.7 [95%置信区间（CI）2.2 - 6.3]，并且与加合物水平存在剂量反应关系。在每天食用水果或蔬菜份数较少的受试者中（对于0 - 1份蔬菜，OR为7.80，95% CI为3.0 - 20.30），病例/对照状态与白细胞DNA加合物存在之间的关联明显强于大量食用者（对于每天食用2份的消费者，OR为4.98；对于食用≥3份的消费者，OR为1.97；对于水果摄入量，发现了类似但较低的估计值）。未发现吸烟与白细胞DNA加合物之间存在关联。在所考虑的几种基因型中，只有NAT - 2与膀胱癌风险（OR为1.72，95% CI为1.03 - 2.87）以及白细胞DNA加合物水平存在统计学上的显著关联。我们的报告表明，水果和蔬菜可能通过抑制DNA加合物的形成来预防膀胱癌。
