O'Sullivan Justin M, Jenkinson Howard F, Cannon Richard D
Department of Oral Sciences and Orthodontics, University of Otago, PO Box 647, Dunedin, New Zealand1.
Microbiology (Reading). 2000 Jan;146 ( Pt 1):41-48. doi: 10.1099/00221287-146-1-41.
Adhesion of Candida albicans to saliva-coated surfaces is an important early step in the colonization of the oral cavity. C. albicans cells also adhere to several species of oral streptococci including Streptococcus gordonii, Streptococcus oralis and Streptococcus sanguinis in what are believed to be multi-modal interactions. It is now demonstrated that incubation of streptococcal cells of these species with human parotid saliva further promotes the adhesion of C. albicans cells by up to 2-3-fold. Various species of streptococci were shown to adsorb different protein components of parotid saliva to their cell surfaces. The basic proline-rich proteins (bPRPs), to which C. albicans cells bind on nitrocellulose blot overlay, were strongly adsorbed to the surface of S. gordonii cells but not to S. oralis cells. Parotid saliva that was pre-adsorbed with S. gordonii cells and then applied to hydroxylapatite beads was <50% effective at supporting adhesion of C. albicans compared with control (non-adsorbed) saliva, demonstrating that bPRPs are major pellicle receptors. C. albicans cells did not adsorb bPRPs from fluid-phase parotid saliva. Following size-exclusion chromatography of parotid saliva samples, pooled fractions enriched in bPRPs promoted maximal adhesion of C. albicans to S. gordonii cells. The results demonstrate that C. albicans cells recognize only surface-bound forms of bPRPs and suggest that these proteins adsorbed to enamel or to streptococcal surfaces promote C. albicans adhesion and oral colonization.
白色念珠菌黏附于唾液包被的表面是其在口腔定植的重要早期步骤。白色念珠菌细胞还会黏附于多种口腔链球菌，包括戈登链球菌、口腔链球菌和血链球菌，这些黏附被认为是多模式相互作用。现已证明，将这些菌种的链球菌细胞与人腮腺唾液一起孵育，可使白色念珠菌细胞的黏附力增强2至3倍。研究表明，不同种类的链球菌会将腮腺唾液的不同蛋白质成分吸附到其细胞表面。白色念珠菌细胞在硝酸纤维素印迹覆盖实验中与之结合的富含脯氨酸的碱性蛋白（bPRPs），被强烈吸附到戈登链球菌细胞表面，而未吸附到口腔链球菌细胞表面。预先用戈登链球菌细胞吸附然后应用于羟基磷灰石珠的腮腺唾液，与对照（未吸附）唾液相比，在支持白色念珠菌黏附方面的效果不到50%，这表明bPRPs是主要的获得性膜受体。白色念珠菌细胞不会从液相腮腺唾液中吸附bPRPs。对腮腺唾液样本进行尺寸排阻色谱分析后，富含bPRPs的合并级分可促进白色念珠菌对戈登链球菌细胞的最大黏附。结果表明，白色念珠菌细胞仅识别表面结合形式的bPRPs，并表明这些吸附在牙釉质或链球菌表面的蛋白质可促进白色念珠菌的黏附和口腔定植。
