Scannapieco F A, Torres G I, Levine M J
Department of Oral Biology, School of Dental Medicine, State University of New York at Buffalo 14214, USA.
J Dent Res. 1995 Jul;74(7):1360-6. doi: 10.1177/00220345950740070701.
Recent studies have demonstrated that several species of oral streptococci, such as Streptococcus gordonii, bind soluble salivary alpha-amylase. The goal of the present study was to determine if amylase immobilized onto a surface such as hydroxyapatite can serve as an adhesion receptor for S. gordonii. Initially, human parotid saliva was fractionated on Bio-Gel P60, and fractions were screened for their ability to promote adhesion of S. gordonii to hydroxyapatite. Fractions containing alpha-amylase and proline-rich proteins promoted the adhesion of [3H]-labeled S. gordonii to hydroxyapatite. Similar findings were obtained with purified amylase and acidic proline-rich protein 1 (PRP1). Incubation of S. gordonii G9B in the presence of starch and maltotriose increased the binding of this strain to amylase-coated hydroxyapatite, while the adhesion of S. sanguis 10556 to amylase-coated hydroxyapatite was not affected by these saccharides. These results suggest that amylase may serve as a hydroxyapatite pellicle receptor for amylase-binding streptococci. Furthermore, starch and starch metabolites may enhance the adhesion of amylase-binding streptococci to amylase in dental pellicles to augment the formation of dental plaque.
最近的研究表明，几种口腔链球菌，如戈登氏链球菌，能够结合可溶性唾液α-淀粉酶。本研究的目的是确定固定在羟基磷灰石等表面的淀粉酶是否可以作为戈登氏链球菌的粘附受体。最初，人腮腺唾液在Bio-Gel P60上进行分级分离，然后筛选各组分促进戈登氏链球菌粘附到羟基磷灰石上的能力。含有α-淀粉酶和富含脯氨酸的蛋白质的组分促进了[3H]标记的戈登氏链球菌对羟基磷灰石的粘附。用纯化的淀粉酶和酸性富含脯氨酸的蛋白质1（PRP1）也得到了类似的结果。在淀粉和麦芽三糖存在的情况下培养戈登氏链球菌G9B，增加了该菌株与包被淀粉酶的羟基磷灰石的结合，而血链球菌10556对包被淀粉酶的羟基磷灰石的粘附不受这些糖类的影响。这些结果表明，淀粉酶可能作为淀粉酶结合链球菌的羟基磷灰石薄膜受体。此外，淀粉和淀粉代谢产物可能增强淀粉酶结合链球菌对牙菌斑中淀粉酶的粘附，从而促进牙菌斑的形成。
