Holmes A R, Gopal P K, Jenkinson H F
Department of Oral Biology and Oral Pathology, University of Otago, Dunedin, New Zealand.
Infect Immun. 1995 May;63(5):1827-34. doi: 10.1128/iai.63.5.1827-1834.1995.
Candida albicans ATCC 10261 and CA2 bound to cells of the oral bacteria Streptococcus gordonii, Streptococcus oralis, and Streptococcus sanguis when these bacteria were immobilized onto microtiter plate wells, but they did not bind to cells of Streptococcus mutans or Streptococcus salivarius. Cell wall polysaccharide was extracted with alkali from S. gordonii NCTC 7869, the streptococcal species to which C. albicans bound with highest affinity, and was effective in blocking the coaggregation of C. albicans and S. gordonii cells in the fluid phase. When fixed to microtiter plate wells, the S. gordonii polysaccharide was bound by all strains of C. albicans tested. The polysaccharide contained Rha, Glc, GalNAc, GlcNAc, and Gal and was related compositionally to previously characterized cell wall polysaccharides from strains of S. oralis and S. sanguis. The adherence of yeast cells to the immobilized polysaccharide was not inhibitable by a number of saccharides. Antiserum raised to the S. gordonii NCTC 7869 polysaccharide blocked adherence of C. albicans ATCC 10261 to the polysaccharide. The results identify a complex cell wall polysaccharide of S. gordonii as the coaggregation receptor for C. albicans. Adherent interactions of yeast cells with streptococci and other bacteria may be important for colonization of both hard and soft oral surfaces by C. albicans.
当白色念珠菌ATCC 10261和CA2固定在微量滴定板孔上时，它们能与口腔细菌戈登链球菌、口腔链球菌和血链球菌的细胞结合，但不与变形链球菌或唾液链球菌的细胞结合。从戈登链球菌NCTC 7869（白色念珠菌与之结合亲和力最高的链球菌种类）中用碱提取细胞壁多糖，该多糖能有效阻断液相中白色念珠菌和戈登链球菌细胞的共聚集。当固定在微量滴定板孔上时，戈登链球菌多糖能被所有测试的白色念珠菌菌株结合。该多糖含有鼠李糖、葡萄糖、N - 乙酰半乳糖胺、N - 乙酰葡萄糖胺和半乳糖，其组成与先前鉴定的口腔链球菌和血链球菌菌株的细胞壁多糖相关。酵母细胞对固定化多糖的黏附不能被多种糖类抑制。针对戈登链球菌NCTC 7869多糖产生的抗血清能阻断白色念珠菌ATCC 10261对该多糖的黏附。结果表明，戈登链球菌的一种复杂细胞壁多糖是白色念珠菌的共聚集受体。酵母细胞与链球菌及其他细菌的黏附相互作用可能对白色念珠菌在口腔软硬表面的定植很重要。
