Minina T S, Andreevskaia E A, Domaradskiĭ I V
Zh Mikrobiol Epidemiol Immunobiol. 1978 Oct(10):61-6.
Use of E. coli strains with phenotypes Rec+ and Rec- asrecipients in intergenera crosses confirmed the supposition put forward by the authors formerly that new chromosomal markers in transconjugantes originated due to Psuedomonas aeruginosa. These chromosomal markers were transferred together with plasmid R conditioning the conjugation, and maintained without being built-into E. coli chromosome. Between the arg+ marker and the plasmid R18 there existed labile physical connection demonstrable only under definite conditions of recombinant selection.
使用具有Rec+和Rec-表型的大肠杆菌菌株作为属间杂交的受体,证实了作者先前提出的假设,即转接合子中的新染色体标记源自铜绿假单胞菌。这些染色体标记与调节接合作用的质粒R一起转移,并在不整合到大肠杆菌染色体的情况下得以维持。在arg+标记和质粒R18之间存在不稳定的物理连接,仅在特定的重组选择条件下才能证明。