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铜绿假单胞菌中一种R'质粒的分离与鉴定

Isolation and characterization of an R' plasmid in Pseudomonas aeruginosa.

作者信息

Holloway B W

出版信息

J Bacteriol. 1978 Mar;133(3):1078-82. doi: 10.1128/jb.133.3.1078-1082.1978.

Abstract

An R' plasmid, R'PA1, carrying a 3- to 4-min segment of the Pseudomonas aeruginosa chromosome has been derived from the incP-1 plasmid R68.45. The chromosomal segment includes the markers argA, argB, argH, and lys-12. The plasmid retains all the properties of R68.45, including chromosome mobilization ability and wide bacterial host range. R'PA1 reverts to R68.45 in rec+ strains of P. aeruginosa, but it can be maintained in a recA strain.

摘要

一个携带铜绿假单胞菌染色体3至4分钟片段的R'质粒R'PA1,是从IncP-1质粒R68.45衍生而来的。该染色体片段包括标记基因argA、argB、argH和lys-12。该质粒保留了R68.45的所有特性,包括染色体动员能力和广泛的细菌宿主范围。R'PA1在铜绿假单胞菌的rec+菌株中可回复为R68.45,但它可以在recA菌株中维持。

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