Parmley R T, Spicer S S, Morgan S K, Grush O C
Cancer. 1976 Sep;38(3):1188-98. doi: 10.1002/1097-0142(197609)38:3<1188::aid-cncr2820380320>3.0.co;2-c.
The ultrastructual and immunologic features of the initial Reed-Sternberg and Hodgkin cells are compared with the ultimate leukemic cell type in a child with Hodgkin's disease who subsequently developed acute myelomonocytic leukemia (AMML) following 29 months of chemotherapy. Hodgkin tumor cells contained cytoplasmic IgG and ultrastructurally resembled large immunoblasts, containing one or two round nuclei with large bizarre nucleoli, many polyribosomes, sparase endoplasmic reticulum, underdeveloped Golgi lamellae, and few cytoplasmic granules. The Hodgkin tumor cells displayed no evidence of phagocytosis. The leukemic monocytic cells did not contain cytoplasmic IgG and, ultrastrucally, exhibited and indented and irregular nuclear profile with less prominent nucleoli, numerous pleomorphic granules, a moderate number of free ribosomes, short segments of endoplasmic reticulum, and stacked Golgi lamellae. The cell surface was irregular and occasionally appeared involved in endocytic activity. These results indicate that the Hodgkin tumor cells originated from B lymphocytes rather than tissue macrophages, whereas the leukemic monocytes arose from the bone marrow-derived monocyte-macrophage series. The findings suggest further that AMML developing after Hodgkin's disease consitutes a second neoplasm rather than a leukemic transformation of Hodgkin tumor cells.
对一名患霍奇金病的儿童进行了29个月化疗后发展为急性粒单核细胞白血病（AMML），将其最初的里德-斯腾伯格细胞和霍奇金细胞的超微结构及免疫特征与最终的白血病细胞类型进行了比较。霍奇金肿瘤细胞含有细胞质IgG，超微结构上类似于大型免疫母细胞，含有一个或两个圆形核，核仁大且形态怪异，有许多多核糖体、稀疏的内质网、发育不全的高尔基片层，细胞质颗粒很少。霍奇金肿瘤细胞没有吞噬作用的证据。白血病单核细胞不含细胞质IgG，超微结构上表现为核轮廓凹陷且不规则，核仁不太明显，有许多多形性颗粒、中等数量的游离核糖体、内质网短片段和堆叠的高尔基片层。细胞表面不规则，偶尔似乎参与内吞活动。这些结果表明，霍奇金肿瘤细胞起源于B淋巴细胞而非组织巨噬细胞，而白血病单核细胞起源于骨髓来源的单核细胞-巨噬细胞系列。这些发现进一步表明，霍奇金病后发生的AMML构成第二种肿瘤，而非霍奇金肿瘤细胞的白血病转化。
