Portakal O, Inal-Erden M
Department of Biochemistry, Osmangazi University, Medical School, Eskişehir, Turkey.
Clin Biochem. 1999 Aug;32(6):461-6. doi: 10.1016/s0009-9120(99)00041-7.
We examined whether pentoxifylline (PTX) and coenzyme Q10 (Q) pretreatment affect ischemia-reperfusion damage in the rat liver.
Twenty minutes of reflow following 30 min of ischemia was performed. Before the experiment, rats were treated PTX 50 mg/kg, IP or PTX 50 mg/kg IP + Q10 mg/kg, intragastric, or untreated. Rats were divided into four groups: control (C), ischemia-reperfusion (IR), PTX-treated (P), and Q+PTX-treated (QP) groups. Hepatic glutathione (GSH) and malondialdehyde (MDA) levels and catalase, superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and reductase (GSSGR) activities were measured.
In IR group GSH levels decreased (p<0.01), conversely MDA levels increased (p<0.01). PTX pretreatment did not affect GSH and MDA values, but Q+PTX pretreatment improved of those (p<0.01). It was shown that catalase and GSH-Px activities increased during ischemia-reperfusion (p<0.01, both of), but PTX pretreatment did not significantly ameliorate those activities. GSSGR activity was higher in IR group than in basal levels (p<0.01). The decrease GSSGR activity that was observed in P group was not significant compared to IR group. During ischemia/reperfusion also SOD activity increased as compared with controls (p<0.05). In PTX-treated group, SOD activity was significantly higher than control and ischemia/reperfusion groups (p<0.01, both of). Q+PTX treatment ameliorated those enzyme activities to the control values.
Short-term hepatic ischemia-reperfusion diminished GSH, increased MDA levels and induced some antioxidant enzyme activities. Q+PTX pretreatment was useful in hepatic ischemia-reperfusion injury, but treatment of PTX alone did not cause beneficial effect in the present study.
我们研究了己酮可可碱（PTX）和辅酶Q10（Q）预处理是否会影响大鼠肝脏的缺血再灌注损伤。
在30分钟缺血后进行20分钟再灌注。实验前，大鼠腹腔注射50mg/kg PTX，或腹腔注射50mg/kg PTX + 灌胃给予10mg/kg Q10，或不进行处理。大鼠分为四组：对照组（C）、缺血再灌注组（IR）、PTX处理组（P）和Q + PTX处理组（QP）。测量肝脏谷胱甘肽（GSH）和丙二醛（MDA）水平以及过氧化氢酶、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）和还原酶（GSSGR）活性。
IR组GSH水平降低（p<0.01），相反MDA水平升高（p<0.01）。PTX预处理不影响GSH和MDA值，但Q + PTX预处理改善了这些值（p<0.01）。结果显示，缺血再灌注期间过氧化氢酶和GSH-Px活性增加（两者均p<0.01），但PTX预处理并未显著改善这些活性。IR组GSSGR活性高于基础水平（p<0.01）。与IR组相比，P组观察到的GSSGR活性降低不显著。与对照组相比，缺血/再灌注期间SOD活性也增加（p<0.05）。在PTX处理组中，SOD活性显著高于对照组和缺血/再灌注组（两者均p<0.01）。Q + PTX处理将这些酶活性改善至对照值。
短期肝脏缺血再灌注降低了GSH水平，增加了MDA水平并诱导了一些抗氧化酶活性。Q + PTX预处理对肝脏缺血再灌注损伤有效，但在本研究中单独使用PTX治疗未产生有益效果。
