Premanathan M, Arakaki R, Izumi H, Kathiresan K, Nakano M, Yamamoto N, Nakashima H
Department of Microbiology and Immunology, Kagoshima University Dental School, Japan.
Antiviral Res. 1999 Dec 15;44(2):113-22. doi: 10.1016/s0166-3542(99)00058-3.
A polysaccharide extracted from the leaf of Rhizophora apiculata (RAP) was assessed in cell culture systems, for its activity against human and simian immunodeficiency viruses. RAP inhibited HIV-1 or HIV-2 or SIV strains in various cell cultures and assay systems. It blocked the expression of HIV-1 antigen in MT-4 cells and abolished the production of HIV-1 p24 antigen in peripheral blood mononuclear cells (PBMC); the 50% effective concentration (EC50) of RAP in HIV-1 infected MT-4 cells and in PBMC was 10.7 and 25.9 microg/ml, respectively. RAP (100 microg/ml) completely blocked the binding of HIV-1 virions to MT-4 cells. RAP also reduced the production of viral mRNA when added before virus adsorption. RAP inhibited syncytium formation in cocultures of MOLT-4 cells and MOLT-4/HIV-1(IIIB) cells. RAP did not prolong activated partial thromboplastin time (APTT) up to 500 microg/ml. These properties may be advantageous should RAP be considered for further development.
对从红树（Rhizophora apiculata）叶片中提取的一种多糖（RAP）在细胞培养系统中进行了评估，以检测其对人类和猿猴免疫缺陷病毒的活性。RAP在各种细胞培养和检测系统中均能抑制HIV-1、HIV-2或SIV毒株。它能阻断MT-4细胞中HIV-1抗原的表达，并消除外周血单个核细胞（PBMC）中HIV-1 p24抗原的产生；在HIV-1感染的MT-4细胞和PBMC中，RAP的50%有效浓度（EC50）分别为10.7和25.9微克/毫升。RAP（100微克/毫升）能完全阻断HIV-1病毒粒子与MT-4细胞的结合。在病毒吸附前加入RAP还能减少病毒mRNA的产生。RAP能抑制MOLT-4细胞与MOLT-4/HIV-1（IIIB）细胞共培养时的合胞体形成。在浓度高达500微克/毫升时，RAP不会延长活化部分凝血活酶时间（APTT）。如果考虑对RAP进行进一步开发，这些特性可能具有优势。
