A polymorphism in the mouse crg-2/IP-10 gene complicates chemokine gene expression analysis using a commercial ribonuclease protection assay.
作者信息
Hallensleben W, Biro L, Sauder C, Hausmann J, Asensio V C, Campbell I L, Staeheli P
机构信息
Abteilung Virologie, Institut für Medizinische Mikrobiologie and Hygiene, Universität Freiburg, Hermann-Herder-Strasse 11, Freiburg, Germany.
出版信息
J Immunol Methods. 2000 Feb 3;234(1-2):149-51. doi: 10.1016/s0022-1759(99)00197-0.
The use of multiprobe RPAs is becoming an increasingly popular method for the detection and quantitation of RNA levels in cells and tissues. Here we report that due to a polymorphism in the 3'-noncoding region of the mouse Crg-2/IP-10 gene, the mCK-5 chemokine probe set available from Pharmingen can yield aberrant signal patterns with RNA samples from BALB/c, MRL and possibly other mouse strains that may lead to false conclusions regarding expression of the Crg-2/IP-10 and MCP-1 genes.
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