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小鼠crg-2/IP-10基因中的一种多态性使使用商业核糖核酸酶保护分析进行趋化因子基因表达分析变得复杂。

A polymorphism in the mouse crg-2/IP-10 gene complicates chemokine gene expression analysis using a commercial ribonuclease protection assay.

作者信息

Hallensleben W, Biro L, Sauder C, Hausmann J, Asensio V C, Campbell I L, Staeheli P

机构信息

Abteilung Virologie, Institut für Medizinische Mikrobiologie and Hygiene, Universität Freiburg, Hermann-Herder-Strasse 11, Freiburg, Germany.

出版信息

J Immunol Methods. 2000 Feb 3;234(1-2):149-51. doi: 10.1016/s0022-1759(99)00197-0.

DOI:10.1016/s0022-1759(99)00197-0
PMID:10669779
Abstract

The use of multiprobe RPAs is becoming an increasingly popular method for the detection and quantitation of RNA levels in cells and tissues. Here we report that due to a polymorphism in the 3'-noncoding region of the mouse Crg-2/IP-10 gene, the mCK-5 chemokine probe set available from Pharmingen can yield aberrant signal patterns with RNA samples from BALB/c, MRL and possibly other mouse strains that may lead to false conclusions regarding expression of the Crg-2/IP-10 and MCP-1 genes.

摘要

多探针核糖核酸酶保护分析（RPA）正日益成为检测和定量细胞及组织中RNA水平的常用方法。我们在此报告，由于小鼠Crg-2/IP-10基因3'-非编码区存在多态性，Pharmingen公司提供的mCK-5趋化因子探针组可能会与BALB/c、MRL以及其他可能的小鼠品系的RNA样本产生异常信号模式，从而可能导致关于Crg-2/IP-10和MCP-1基因表达的错误结论。

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