Blauer G, Akkawi M
Department of Biological Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Biochem J. 2000 Mar 1;346 Pt 2(Pt 2):249-50.
Synthetic beta-haematin is considered to be identical, or at least very similar to, purified malaria pigment. Methods for its preparation use ferriprotoporphyrin IX at acid pH in the presence of acetic acid at different concentrations and degrees of ionization, elevated temperatures and long reaction times. Here we show that certain widely used reaction conditions, involving very high concentrations of acetic acid/acetate mixtures, do not produce substantial amounts of polymeric beta-haematin on immediate isolation of the reaction products, but only during prolonged drying of the products at 37 degrees C after washing with water. Alternative, more convenient methods of preparation of pure beta-haematin are suggested.
合成β-血红素被认为与纯化的疟色素相同，或至少非常相似。其制备方法是在不同浓度和电离度的乙酸存在下，于酸性pH条件下使用高铁原卟啉IX，同时需要高温和较长的反应时间。我们在此表明，某些广泛使用的反应条件，即涉及非常高浓度的乙酸/乙酸盐混合物，在立即分离反应产物时不会产生大量的聚合β-血红素，而是仅在用水洗涤后于37℃长时间干燥产物的过程中产生。本文还提出了制备纯β-血红素的其他更简便方法。
