Quantitative imaging of pre-mRNA splicing factors in living cells.
作者信息
Eils R, Gerlich D, Tvaruskó W, Spector D L, Misteli T
机构信息
Bioinformatics Group, Interdisciplinary Center of Scientific Computing, University of Heidelberg, 69120 Heidelberg, Germany.
出版信息
Mol Biol Cell. 2000 Feb;11(2):413-8. doi: 10.1091/mbc.11.2.413.
Abstract
摘要
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本文引用的文献
1
RNA polymerase II targets pre-mRNA splicing factors to transcription sites in vivo.RNA聚合酶II在体内将前体mRNA剪接因子靶向转录位点。
Mol Cell. 1999 Jun;3(6):697-705. doi: 10.1016/s1097-2765(01)80002-2.
2
Time-resolved analysis and visualization of dynamic processes in living cells.活细胞中动态过程的时间分辨分析与可视化
Proc Natl Acad Sci U S A. 1999 Jul 6;96(14):7950-5. doi: 10.1073/pnas.96.14.7950.
3
Serine phosphorylation of SR proteins is required for their recruitment to sites of transcription in vivo.SR蛋白的丝氨酸磷酸化是其在体内被募集到转录位点所必需的。
J Cell Biol. 1998 Oct 19;143(2):297-307. doi: 10.1083/jcb.143.2.297.
4
The cellular organization of gene expression.基因表达的细胞组织
Curr Opin Cell Biol. 1998 Jun;10(3):323-31. doi: 10.1016/s0955-0674(98)80007-0.
5
Structure and function in the nucleus.细胞核的结构与功能
Science. 1998 Apr 24;280(5363):547-53. doi: 10.1126/science.280.5363.547.
6
Interphase chromosomes undergo constrained diffusional motion in living cells.间期染色体在活细胞中进行受限的扩散运动。
Curr Biol. 1997 Dec 1;7(12):930-9. doi: 10.1016/s0960-9822(06)00412-x.
7
Applications of the green fluorescent protein in cell biology and biotechnology.绿色荧光蛋白在细胞生物学和生物技术中的应用。
Nat Biotechnol. 1997 Oct;15(10):961-4. doi: 10.1038/nbt1097-961.
8
The dynamics of a pre-mRNA splicing factor in living cells.活细胞中前体mRNA剪接因子的动力学
Nature. 1997 May 29;387(6632):523-7. doi: 10.1038/387523a0.
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Three-dimensional reconstruction of painted human interphase chromosomes: active and inactive X chromosome territories have similar volumes but differ in shape and surface structure.绘制的人类间期染色体的三维重建：活跃和不活跃的X染色体区域体积相似，但形状和表面结构不同。
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