Purdom C E, Thompson D, Dando P R
Heredity (Edinb). 1976 Oct;37(2):193-206. doi: 10.1038/hdy.1976.82.
Genetic analysis was performed on five enzyme systems (G3PDH; GPI-A; GPI-B; PGM; MDH-A) in plaice (Pleuronectes platessa) collected in spawning condition from the North Sea. Conventional crosses, induced gynogenesis and induced triploidy were performed. The data conclusively demonstrated the inheritance of isozymes by co-dominant alleles at individual loci for each system. No linkage was observed but tests did not include MDH nor the possibility of linkage between G3PDH and GPI-A. Some anomalous segregation ratios were observed, particularly a deficiency of heterozygotes for GPI-A, but the data were largely in conformity with Mendelian expectations. At the PGM locus, five independent anomalous individuals were scored and interpreted as mutations with a mutation rate of 1.1 X 10(-3) per gamete. Recombination with the centromere was assessed and induced triploidy and cross-over values of 41 per cent for PGM, 19 per cent for MDH-A and 9 per cent for GPI-B were derived on the assumption of complete interference. Amongst the parent fish, genotypic and phenotypic frequencies were largely consistent with the expectations of the Hardy-Weinberg Law, and allelic frequencies were not significantly different between year of collection or location of collection ground.
对从北海采集的处于产卵状态的鲽（Pleuronectes platessa）的五个酶系统（G3PDH；GPI - A；GPI - B；PGM；MDH - A）进行了遗传分析。进行了常规杂交、诱导雌核发育和诱导三倍体实验。数据确凿地证明了每个系统中各个位点的共显性等位基因对等位酶的遗传。未观察到连锁现象，但测试未包括MDH以及G3PDH和GPI - A之间存在连锁的可能性。观察到一些异常的分离比例，特别是GPI - A杂合子的缺乏，但数据在很大程度上符合孟德尔预期。在PGM位点，对五个独立的异常个体进行了评分，并解释为突变，每个配子的突变率为1.1×10⁻³。在完全干扰的假设下，评估了与着丝粒的重组情况，并得出PGM的诱导三倍体和交换值为41%，MDH - A为19%，GPI - B为9%。在亲鱼中，基因型和表型频率在很大程度上符合哈迪 - 温伯格定律的预期，并且等位基因频率在采集年份或采集地点之间没有显著差异。
