噬菌体φX - 174体内信使核糖核酸的定位
Mapping of in vivo messenger RNAs for bacteriophage phiX-174.
作者信息
Hayashi M, Fujimura F K, Hayashi M
出版信息
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3519-23. doi: 10.1073/pnas.73.10.3519.
Abstract
In vivo messenger RNA for bacteriophage phiX174 was fractionated in agarose gels into a number of discrete species ranging in size from 0.23 X 10(6) to 2.3 X 10(6) daltons. These RNA species were eluted from the gels and hybridized to specific fragments derived from phiX-174 replicative form DNA by cleavage with restriction enzymes. A map of the orientation of in vivo messenger RNAs with respect to the bacteriophage genetic map was constructed. This map indicated that initiation of messenger RNA occurred before gene B, gene C or D, and probably before gene A and that termination occurred after genes E, F, G, and H. Termination of transcription at any particular site appeared not to be entirely effective such that a number of overlapping transcripts with the same 5'-terminus was observed. Messenger RNA for gene A seemed to be relatively unstable.
摘要
噬菌体φX174的体内信使核糖核酸（mRNA）在琼脂糖凝胶中被分离成许多大小从0.23×10⁶到2.3×10⁶道尔顿不等的离散种类。这些RNA种类从凝胶中洗脱出来，并与通过限制性酶切从φX - 174复制型DNA获得的特定片段杂交。构建了体内信使RNA相对于噬菌体遗传图谱的方向图。该图谱表明信使RNA的起始发生在基因B、基因C或D之前，可能也在基因A之前，而终止发生在基因E、F、G和H之后。在任何特定位点的转录终止似乎并不完全有效，以至于观察到许多具有相同5' - 末端的重叠转录本。基因A的信使RNA似乎相对不稳定。
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