Horiuchi M, Yamano S, Inoue H, Ishii J, Nagata Y, Adachi H, Ono M, Renard J N, Mizuno F, Hayashi Y, Saito I
Department of Microbiology, Tokyo Medical University, Japan.
J Clin Pathol. 1999 Nov;52(11):833-7. doi: 10.1136/jcp.52.11.833.
To determine the correlation between interleukin 12 (IL-12) expression and Epstein-Barr virus (EBV) in Sjögren syndrome.
Indirect immunohistochemical technique, enzyme linked immunosorbent assay (ELISA), and immunoblot analysis were used to investigate IL-12 expression by EBV activation, using 13 surgical specimens and four B cell lines.
Marked expression of IL-12 was found in the epithelial cells and the infiltrating B cells of salivary gland tissues from patients with Sjögren syndrome (six of 10 cases), but not in those from normal individuals (none of three cases). A striking topographic correlation between IL-12 and EBV was found. In addition, levels of IL-12 production by B cell lines were clearly enhanced by EBV activation in vitro.
IL-12 expression closely reflects the intracellular event of EBV activation in Sjögren syndrome, and may contribute to the T helper cell type 1 (Th1) cytokine overexpression seen in this disease.
确定干燥综合征中白细胞介素12（IL-12）表达与爱泼斯坦-巴尔病毒（EBV）之间的相关性。
采用间接免疫组织化学技术、酶联免疫吸附测定（ELISA）和免疫印迹分析，利用13份手术标本和4种B细胞系，研究EBV激活对IL-12表达的影响。
在干燥综合征患者唾液腺组织的上皮细胞和浸润性B细胞中发现IL-12有明显表达（10例中有6例），而正常个体唾液腺组织中未发现（3例均无）。发现IL-12与EBV之间存在显著的拓扑相关性。此外，体外EBV激活可明显增强B细胞系产生IL-12的水平。
IL-12表达密切反映干燥综合征中EBV激活的细胞内事件，并可能导致该疾病中所见的1型辅助性T细胞（Th1）细胞因子过表达。
