De La Rúa P, Simon U E, Tilde A C, Moritz R F, Fuchs S
Institut Zoologie-Molekulare Okologie, Martin Luther Universität Halle/Wittenberg, Kröllwitzerstr. 44, D-06099 Halle/Saale, Germany.
Heredity (Edinb). 2000 Jan;84 ( Pt 1):124-30. doi: 10.1046/j.1365-2540.2000.00646.x.
The cavity-nesting honeybee Apis cerana occurs in Asia, from Afghanistan to China and from Japan to southern Indonesia. Based on morphometric values, this species can be grouped into four subspecies: A. c. cerana, A. c. indica, A. c. japonica and A. c. himalaya. In order to analyse the geographical variability of A. c. indica from the Philippine Islands, 47 colonies from different locations in three of the larger islands (Mindanao, Luzon and Palawan) and four of the Visayan Islands (Panay, Negros, Cebu and Leyte) were studied. Genetic variation was estimated by restriction and sequence analysis of PCR-amplified fragments of the tRNAleu-COII region. We found four different haplotypes, Ce1, Ce2, Ce3 and Ce4, that discriminate among the bee populations from different islands. The Ce1 haplotype is present in Mindanao and Visayan Islands, Ce2 is restricted to Luzon, and both Ce3 and Ce4 are only present in Palawan. Phylogenetic analysis of the sequences shows a great intraspecific variability, is in accordance with the geological history of these islands and partially agrees with some previous morphological and molecular studies.
营巢于洞穴的东方蜜蜂分布在亚洲，从阿富汗到中国，从日本到印度尼西亚南部。根据形态测量值，该物种可分为四个亚种：中华蜜蜂指名亚种、印度蜜蜂、日本蜜蜂和喜马拉雅蜜蜂。为了分析来自菲律宾群岛的印度蜜蜂的地理变异性，我们研究了来自三个较大岛屿（棉兰老岛、吕宋岛和巴拉望岛）以及四个米沙鄢群岛（班乃岛、内格罗斯岛、宿务岛和莱特岛）不同地点的47个蜂群。通过对tRNAleu - COII区域的PCR扩增片段进行限制性分析和序列分析来估计遗传变异。我们发现了四种不同的单倍型，Ce1、Ce2、Ce3和Ce4，它们能够区分来自不同岛屿的蜜蜂种群。Ce1单倍型存在于棉兰老岛和米沙鄢群岛，Ce2仅限于吕宋岛，而Ce3和Ce4仅存在于巴拉望岛。对这些序列的系统发育分析显示出很大的种内变异性，这与这些岛屿的地质历史一致，并且部分与之前的一些形态学和分子研究结果相符。
