Oehen S, Junt T, López-Macías C, Kramps T A
University Hospital Zürich, Institute for Experimental Immunology, Zürich, Switzerland.
Immunology. 2000 Feb;99(2):163-9. doi: 10.1046/j.1365-2567.2000.00950.x.
In this study, we investigated the potential of a DNA vaccine expressing the minimal cytotoxic T lymphocyte (CTL) epitope gp33 of the lymphocytic choriomeningitis virus glycoprotein to protect against infection of a non-lymphoid organ and compared this to protection against a systemic infection. Furthermore, since immune stimulatory sequences have been shown to augment CTL responses, we examined the capacity of CpG DNA to enhance CTL memory. The data show that DNA vaccination with a gp33-based gene construct induced short-lived gp33-specific CTL which protected against a systemic infection but not against a peripheral infection. Immune stimulatory sequences were incapable of either prolonging CTL memory or promoting protection against infection of a peripheral organ.
在本研究中，我们调查了一种表达淋巴细胞性脉络丛脑膜炎病毒糖蛋白最小细胞毒性T淋巴细胞（CTL）表位gp33的DNA疫苗预防非淋巴器官感染的潜力，并将其与预防全身感染的效果进行了比较。此外，由于免疫刺激序列已被证明可增强CTL反应，我们检测了CpG DNA增强CTL记忆的能力。数据显示，用基于gp33的基因构建体进行DNA疫苗接种可诱导短暂的gp33特异性CTL，其可预防全身感染，但不能预防外周感染。免疫刺激序列既不能延长CTL记忆，也不能促进对外周器官感染的预防。
