Larsen D L, Dybdahl-Sissoko N, McGregor M W, Drape R, Neumann V, Swain W F, Lunn D P, Olsen C W
Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin-Madison, 53706, USA.
J Virol. 1998 Feb;72(2):1704-8. doi: 10.1128/JVI.72.2.1704-1708.1998.
This study was conducted to investigate whether Accell gene gun coadministration of DNA encoding human interleukin-6 (IL-6) would enhance protective immune responses in mice to an equine influenza A virus hemagglutinin (HA) DNA vaccine. Mice that received HA DNA alone exhibited accelerated clearance of homologous challenge virus but were not protected from infection. In contrast, mice that received both HA and IL-6 DNA had no detectable virus in their lungs after challenge. These results strongly support the use of IL-6 as a cytokine adjuvant in DNA vaccination.
本研究旨在调查通过Accell基因枪共同给予编码人白细胞介素-6（IL-6）的DNA是否会增强小鼠对马甲型流感病毒血凝素（HA）DNA疫苗的保护性免疫反应。单独接受HA DNA的小鼠对同源攻击病毒的清除加速，但未免受感染。相比之下，同时接受HA和IL-6 DNA的小鼠在攻击后肺部未检测到病毒。这些结果有力地支持了将IL-6用作DNA疫苗接种中的细胞因子佐剂。
