Bakhtiar R, Nelson R W
Department of Chemistry, Stevens Institute of Technology, Hoboken, NJ, USA.
Biochem Pharmacol. 2000 Apr 15;59(8):891-905. doi: 10.1016/s0006-2952(99)00317-2.
Tremendous progress in biomedical sciences has been made possible in part by recent advances in bioanalytical methods, in particular biological mass spectrometry. Since the introduction of electrospray ionization mass spectrometry (ESI-MS) in 1984 and matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) in 1988, the field of bioanalytical mass spectrometry has seen rapid growth. In concert with separation techniques such as capillary electrophoresis and high performance liquid chromatography, mass spectrometry allows characterization of a large array of small organic molecules, peptides, proteins, oligonucleotides, and RNA fragments. Thus, substantially more expedient and definitive determination of molecular weight is now possible by mass spectrometric analysis. In this commentary, general descriptions of ESI- and MALDI-MS are presented. Furthermore, several recent developments and applications in addressing difficult biological problems are discussed.
生物医学科学取得的巨大进展部分得益于生物分析方法的最新进展，尤其是生物质谱。自1984年引入电喷雾电离质谱（ESI-MS）和1988年引入基质辅助激光解吸电离质谱（MALDI-MS）以来，生物分析质谱领域发展迅速。与毛细管电泳和高效液相色谱等分离技术相结合，质谱能够对大量的小有机分子、肽、蛋白质、寡核苷酸和RNA片段进行表征。因此，现在通过质谱分析可以更快速、更准确地测定分子量。在这篇评论中，对ESI-MS和MALDI-MS进行了一般性描述。此外，还讨论了在解决棘手生物学问题方面的一些最新进展和应用。
