Uma H, Selvaraj P, Reetha A M, Xavier T, Prabhakar R, Narayanan P R
Tuberculosis Research Centre (ICMR), Chennai, India.
Tuber Lung Dis. 1999;79(4):199-206. doi: 10.1054/tuld.1999.0211.
Influence of HLA-DR antigens and lymphocyte responses in pulmonary TB patients.
To elucidate the role of HLA-DR genes/gene products on lymphocyte responses to Mycobacterium tuberculosis antigens and mitogens, the present study was carried out in pulmonary tuberculosis during active and cured stage of the disease.
Serological determination of HLA-DR antigens was carried out in 50 active TB patients, 44 cured TB patients and 58 normal healthy control subjects. The influence of HLA-DR antigens on peripheral blood lymphocyte responses to M. tuberculosis culture filtrate antigens and mitogens such as phytohaemagglutinin (PHA) and concanavalin-A (Con-A) was studied in the patients as well as normal healthy control subjects.
Of all the DR antigens studied, patients (active TB and cured TB) with DR2 antigen showed an increased lymphocyte response (stimulation index) to a higher dose of antigenic (10 micrograms/ml) stimulation. A significantly lower lymphocyte response to antigen and mitogens was seen in HLA-DR3 positive normal healthy subjects than non-DR3 (DR3 negative) subjects.
The present study suggests that HLA-DR genes/gene products may be playing an immunoregulatory role in eliciting an immune response against M. tuberculosis antigens and mitogens induced lymphocyte response in pulmonary TB patients and normal healthy subjects.
HLA - DR抗原及淋巴细胞反应在肺结核患者中的影响。
为阐明HLA - DR基因/基因产物在淋巴细胞对结核分枝杆菌抗原及丝裂原反应中的作用，本研究在肺结核疾病的活动期和治愈期进行。
对50例活动性肺结核患者、44例治愈肺结核患者及58例正常健康对照者进行HLA - DR抗原的血清学检测。在患者及正常健康对照者中研究HLA - DR抗原对外周血淋巴细胞对结核分枝杆菌培养滤液抗原及丝裂原如植物血凝素（PHA）和刀豆球蛋白A（Con - A）反应的影响。
在所有研究的DR抗原中，携带DR2抗原的患者（活动性肺结核和治愈肺结核）对较高剂量抗原（10微克/毫升）刺激的淋巴细胞反应（刺激指数）增加。HLA - DR3阳性的正常健康受试者对抗原和丝裂原的淋巴细胞反应显著低于非DR3（DR3阴性）受试者。
本研究提示HLA - DR基因/基因产物可能在引发针对结核分枝杆菌抗原的免疫反应以及在肺结核患者和正常健康受试者中诱导丝裂原引起的淋巴细胞反应中发挥免疫调节作用。
