Oettinger T, Jørgensen M, Ladefoged A, Hasløv K, Andersen P
Department of TB Immunology, Statens Serum Institut, Copenhagen, Denmark.
Tuber Lung Dis. 1999;79(4):243-50. doi: 10.1054/tuld.1999.0206.
The original Mycobacterium bovis Bacillus Calmette Guérin vaccine strain has developed into several different substrains which have been used for production of BCG vaccines throughout the world since 1921. Based on the latest genetic and antigenic knowledge, as well as the early literature reports on BCG vaccination, we are able to fit the different pieces of the BCG puzzle together and outline the origin of the different substrains of M. bovis BCG. The BCG vaccine substrains analysed demonstrate two distinct patterns, with an abrupt change consisting of a loss of several genes and altered biochemical characteristics in strains originating from Institut Pasteur after 1927. Further evidence from the literature is provided that a change occurred in virulence of the BCG parent strain at Institut Pasteur in the late 1920s. Based on this information a genealogical tree is proposed and discussed.
最初的牛分枝杆菌卡介苗菌株已发展出几种不同的亚菌株，自1921年以来，这些亚菌株已在全球用于生产卡介苗。基于最新的遗传和抗原知识，以及早期关于卡介苗接种的文献报道，我们能够将卡介苗难题的不同部分拼凑在一起，并勾勒出牛分枝杆菌卡介苗不同亚菌株的起源。所分析的卡介苗疫苗亚菌株呈现出两种不同的模式，1927年后源自巴斯德研究所的菌株发生了突然变化，包括几个基因的缺失和生化特性的改变。文献提供了进一步的证据，表明20世纪20年代末巴斯德研究所的卡介苗亲本菌株的毒力发生了变化。基于这些信息，提出并讨论了一个系谱树。
