线性双链DNA的超结构

Vollenweider H J, Koller T, Parello J, Sogo J M

Proc Natl Acad Sci U S A. 1976 Nov;73(11):4125-9. doi: 10.1073/pnas.73.11.4125.

The superstructure of a covalently closed circular DNA (of bacteriophage PM 2) was compared by electron microscopy with that of a linear duplex DNA (of bacteriophage T7) when ionic strength and benzyldimethylalkylammonium chloride concentration were varied. In parallel studies the sedimentation behavior of these DNAs was studied by analytical ultracentrifugation, but for technical reasons these had to be without benzyldimethylalkylammonium chloride. By combining the information from the two methods one has to conclude that with increasing ionic strength the linear duplex T7 DNA spontaneously forms a structure similar to that of the superhelical structure of closed circular PM 2 DNA. The superstructure is destroyed under premelting conditions and in the presence of an excess of ethidium bromide.

当离子强度和苄基二甲基烷基氯化铵浓度变化时，通过电子显微镜比较了（噬菌体PM 2的）共价闭合环状DNA与（噬菌体T7的）线性双链DNA的超结构。在平行研究中，通过分析超速离心研究了这些DNA的沉降行为，但由于技术原因，这些研究必须在没有苄基二甲基烷基氯化铵的情况下进行。通过结合两种方法获得的信息可以得出结论，随着离子强度的增加，线性双链T7 DNA会自发形成一种与闭合环状PM 2 DNA的超螺旋结构相似的结构。这种超结构在预熔解条件下以及在过量溴化乙锭存在时会被破坏。

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Vollenweider H J, Koller T, Parello J, Sogo J M

Proc Natl Acad Sci U S A. 1976 Nov;73(11):4125-9. doi: 10.1073/pnas.73.11.4125.

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Ethidium bromide-mediated renaturation of denatured closed circular DNAs. The nature of denaturation-resistant fractions of bacteriophage PM2 closed circular DNA.

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Purification of closed circular lambda deoxyribonucleic acid and its sedimentation properties as a function of Sodium chloride concentration and ethidium binding.

J Biol Chem. 1975 Feb 10;250(3):1071-9.

Enumeration of interacting species and sedimentation properties of fd-DNA at low ionic strength.

Biophys Chem. 1974 Feb;1(3):141-51. doi: 10.1016/0301-4622(74)80001-3.

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Arch Biochem Biophys. 1972 Oct;152(2):723-32. doi: 10.1016/0003-9861(72)90268-8.

Sedimentation analysis of conformation changes of circular PM 2 DNA in relation to the ionic strength.

Biochim Biophys Acta. 1971 Dec 30;254(3):407-11. doi: 10.1016/0005-2787(71)90872-0.

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EMBO J. 1983;2(12):2123-8. doi: 10.1002/j.1460-2075.1983.tb01712.x.

Sedimentation velocity behavior of closed circular SV40 DNA as a function of superhelix density, ionic strength, counterion and temperature.

J Mol Biol. 1971 Nov 28;62(1):21-38. doi: 10.1016/0022-2836(71)90128-8.

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Biochemistry. 1978 May 16;17(10):1890-6. doi: 10.1021/bi00603a014.

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Nucleic Acids Res. 1980 Oct 10;8(19):4485-99. doi: 10.1093/nar/8.19.4485.

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Med Microbiol Immunol. 1986;175(1):1-13. doi: 10.1007/BF02123124.

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Virology. 1964 Nov;24:388-92. doi: 10.1016/0042-6822(64)90176-x.

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Biochim Biophys Acta. 1964 Jun 22;87:358-61. doi: 10.1016/0926-6550(64)90238-5.

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J Mol Biol. 1961 Feb;3:18-30. doi: 10.1016/s0022-2836(61)80004-1.

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Sedimentation analysis of the ionic strength dependence of the tertiary structure of native DNA in solution.

Biopolymers. 1971;10(5):827-37. doi: 10.1002/bip.360100507.

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J Mol Biol. 1970 Dec 14;54(2):247-79. doi: 10.1016/0022-2836(70)90429-8.

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J Cell Sci. 1970 Jul;7(1):5-13. doi: 10.1242/jcs.7.1.5.

Direct determination of the superhelix density of closed circular DNA by viscometric titration.

Nat New Biol. 1971 Jan 6;229(1):10-3. doi: 10.1038/newbio229010a0.

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Proc Natl Acad Sci U S A. 1969 Aug;63(4):1164-8. doi: 10.1073/pnas.63.4.1164.

Uncoiling of bacteriophage PM2 DNA by binding of steroidal diamines.

Biochim Biophys Acta. 1972 Feb 23;262(1):18-23. doi: 10.1016/0005-2787(72)90214-6.

Superstructure of linear duplex DNA.

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线性双链DNA的超结构

Superstructure of linear duplex DNA.

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