超螺旋脱氧核糖核酸的构象变化
Conformational variation in superhelical deoxyribonucleic acid.
作者信息
Campbell A M
出版信息
Biochem J. 1978 Apr 1;171(1):281-3. doi: 10.1042/bj1710281.
Abstract
Sedimentation experiments have shown that superhelical DNA undergoes a sharp structural transition at low ionic strength. Light-scattering experiments show that this is due to a change in conformation of the DNA rather than to a change in interactions among DNA molecules. The results show that two possible conformations can occur for superhelical DNA under routine experimental conditions and may explain the discrepancies in the number of early unwinding sites exposed by different techniques.
摘要
沉降实验表明，超螺旋DNA在低离子强度下会发生急剧的结构转变。光散射实验表明，这是由于DNA构象的变化，而不是DNA分子间相互作用的改变。结果表明，在常规实验条件下，超螺旋DNA可能会出现两种构象，这或许可以解释不同技术所揭示的早期解旋位点数量存在差异的原因。
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