Kurtiş B, Develioğlu H, Taner I L, Baloş K, Tekin I O
Department of Periodontology, Faculty of Dentistry, Gazi University, Ankara, Turkey.
J Oral Sci. 1999 Dec;41(4):163-7. doi: 10.2334/josnusd.41.163.
Cytokines play an important role in the pathology associated with chronic inflammatory diseases. One of these cytokines, interleukin 6 (IL-6) is a major mediator of the host response to tissue injury, infection and bone resorption. In the present study, gingival crevicular fluid (GCF) level of IL-6 was determined in patients with non-insulin dependent diabetes mellitus (NIDDM) with periodontitis, adult periodontitis, and healthy controls by use of an enzyme linked immunosorbent assay (ELISA). Twenty-four NIDDM patients with periodontitis, twenty-four adult periodontitis and twenty-four healthy controls were selected for the study. GCF sampling was performed on the vestibular aspects of maxillary incisors and canine teeth. Plaque index (PI), gingival index (GI), gingival bleeding time index (GBTI), probing depth (PD) and probing attachment levels (PAL) were recorded from each sampling area and also the entire dentition. NIDDM and adult periodontitis patients had numerous sites with radiographic evidence of alveolar bone resorption, loss of attachment and pocket depth greater than 3 mm. The mean GCF IL-6 level was 2.43 +/- 0.97 ng/ml in NIDDM patients, 1.31 +/- 0.92 ng/ml in adult periodontitis and 0.62 +/- 0.58 ng/ml in healthy subjects, respectively (p < 0.05). GCF IL-6 levels were markedly higher in NIDDM and adult periodontitis groups compared to the healthy controls. No correlation was found between GCF IL-6 levels and all clinical parameters. These findings suggested that GCF IL-6 levels were significantly higher in the area of inflammation and periodontal destruction locally. The high IL-6 levels in NIDDM patients might be due to different microbial flora in periodontal pockets and altered immune system. Future studies are needed to evaluate the complex interaction among IL-6 GCF levels, host response and local microbial environment in the NIDDM patients.
细胞因子在与慢性炎症性疾病相关的病理学中发挥着重要作用。这些细胞因子之一，白细胞介素6（IL-6）是宿主对组织损伤、感染和骨吸收反应的主要介质。在本研究中，通过酶联免疫吸附测定（ELISA）测定了患有牙周炎的非胰岛素依赖型糖尿病（NIDDM）患者、成人牙周炎患者和健康对照者的龈沟液（GCF）中IL-6的水平。选择了24名患有牙周炎的NIDDM患者、24名成人牙周炎患者和24名健康对照者进行研究。在上颌切牙和尖牙的前庭面进行GCF采样。记录每个采样区域以及整个牙列的菌斑指数（PI）、牙龈指数（GI）、牙龈出血时间指数（GBTI）、探诊深度（PD）和探诊附着水平（PAL）。NIDDM和成人牙周炎患者有许多部位有影像学证据显示牙槽骨吸收、附着丧失和袋深大于3mm。NIDDM患者的平均GCF IL-6水平为2.43±0.97ng/ml，成人牙周炎患者为1.31±0.92ng/ml，健康受试者为0.62±0.58ng/ml（p<0.05）。与健康对照相比，NIDDM组和成人牙周炎组的GCF IL-6水平明显更高。未发现GCF IL-6水平与所有临床参数之间存在相关性。这些发现表明，在炎症和局部牙周破坏区域，GCF IL-6水平显著更高。NIDDM患者中IL-6水平较高可能是由于牙周袋中不同的微生物群落和免疫系统改变所致。未来需要进行研究以评估NIDDM患者中IL-6 GCF水平、宿主反应和局部微生物环境之间的复杂相互作用。
