de Mônico Magalhães M, Garbacio V L, de Almeida M B, Braz A C, Moraes-Santos T, Freire-Maia L, Cunha-Melo J R
Department of Surgery, School of Medicine, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil.
Toxicon. 2000 Jun;38(6):855-64. doi: 10.1016/s0041-0101(99)00202-0.
In the present work the pH and arterial blood gases were measured in fasted and fed male albino rats, weighing 297 +/- 13 g, anaesthetized with urethane (1.4 g/kg, i.p.) before and after injection of T1 fraction from Titys serrulatus scorpion venom, during 60 min. Arterial blood samples were collected at 0, 5, 15, 30 and 60 min for pH, pCO2, pO2, bicarbonate and base-excess analysis. The data showed that the scorpion toxin induced a continuous drop in the blood pH along the time. Hypercapnia and hypoxemia peaking at 30 min and followed by a recovery towards normal values at 60 min were also observed. A pronounced decrease in the blood bicarbonate levels at 60 min and negative base-excess values along with time were evident at 60 min. The comparisons between fasted and fed animals have shown that in the last group the effects of scorpion toxin on the arterial blood gases were less pronounced. We conclude that T1 fraction of Tityus serrulatus scorpion venom induces in anaesthetized rats an acute respiratory acidosis followed by metabolic acidosis.
在本研究中，对体重为297±13克的雄性白化大鼠进行实验，在注射锯尾蝎（Tityus serrulatus）毒液的T1组分前后，分别对禁食和喂食状态的大鼠进行测量，用氨基甲酸乙酯（1.4克/千克，腹腔注射）麻醉，观察60分钟。在0、5、15、30和60分钟时采集动脉血样本，进行pH、pCO₂、pO₂、碳酸氢盐和碱剩余分析。数据显示，蝎毒素会导致血液pH值随时间持续下降。还观察到高碳酸血症和低氧血症在30分钟时达到峰值，随后在60分钟时恢复到正常水平。60分钟时血液碳酸氢盐水平明显下降，60分钟时碱剩余值随时间呈负值。禁食和喂食动物之间的比较表明，在后一组中蝎毒素对动脉血气的影响不太明显。我们得出结论，锯尾蝎毒液的T1组分在麻醉大鼠中会诱发急性呼吸性酸中毒，随后是代谢性酸中毒。
