Lisboa Thiago A, Andrade Marcus Vinícius M, Rezende-Neto João B, Silva Maria Júlia M, Carvalho Mário G, Moraes-Santos Tasso, Ribeiro Daniel D, Cunha-Melo José Renan
Federal University of Minas Gerais, Brazil.
Department of of Internal Medicine, Federal University of Minas Gerais, Brazil.
Toxicon. 2015 Feb;94:45-9. doi: 10.1016/j.toxicon.2014.10.010. Epub 2014 Nov 7.
Thromboelastometry was used to evaluate blood coagulation in anesthetized rats after intravenous administration of Tityus serrulatus scorpion venom (Tx). Tracheostomy followed by catheterization of the left jugular vein and right carotid artery were performed for Tx or Ringer's lactate solution injection and blood sample harvesting, respectively. Blood samples were obtained at the beginning of the experiments (baseline) and at two, five, 15, 30, and 60 min after intoxication. The following coagulation parameters were analyzed: CT (Clotting Time), CFT (Clotting Formation Time), Alpha Angle (α), MCF (Maximum Clot Firmness) and TPI (Thrombodynamic Potential Index). Toxin-induced hypercoagulability was demonstrated at the 15 and 60 min. We hypothesize Tx-induced hypercoagulability and enhanced clot formation could be explained by catecholamine release, systemic inflammatory response, and complement system activation, at least in the first hour after envenomation. Further studies are needed to determine the molecular mechanism of Tx-induced coagulopathy.
采用血栓弹力图法评估静脉注射锯齿蝎毒液（Tx）后麻醉大鼠的血液凝固情况。分别进行气管切开术，随后经左颈静脉插管用于注射Tx或乳酸林格液，经右颈动脉插管用于采集血样。在实验开始时（基线）以及中毒后2、5、15、30和60分钟采集血样。分析以下凝血参数：凝血时间（CT）、凝血形成时间（CFT）、α角（α）、最大血凝块硬度（MCF）和血栓动力学潜能指数（TPI）。在15分钟和60分钟时证实了毒素诱导的高凝状态。我们推测，Tx诱导的高凝状态和血凝块形成增强至少在中毒后的第一个小时内可由儿茶酚胺释放、全身炎症反应和补体系统激活来解释。需要进一步研究以确定Tx诱导的凝血病的分子机制。
