土壤微宇宙中不动杆菌属BD413菌株（pFG4DeltanptII）用转基因植物DNA进行转化及卡那霉素对转化体选择的影响
Transformation of Acinetobacter sp. strain BD413(pFG4DeltanptII) with transgenic plant DNA in soil microcosms and effects of kanamycin on selection of transformants.
作者信息
Nielsen K M, van Elsas J D, Smalla K
机构信息
Unigen and Department of Botany, Norwegian University of Science and Technology, 7491 Trondheim, Norway.
出版信息
Appl Environ Microbiol. 2000 Mar;66(3):1237-42. doi: 10.1128/AEM.66.3.1237-1242.2000.
Abstract
Here we show that horizontal transfer of DNA, extracted from transgenic sugar beets, to bacteria, based on homologous recombination, can occur in soil. Restoration of a 317-bp-deleted nptII gene in Acinetobacter sp. strain BD413(pFG4) cells incubated in sterile soil microcosms was detected after addition of nutrients and transgenic plant DNA encoding a functional nptII gene conferring bacterial kanamycin resistance. Selective effects of the addition of kanamycin on the population dynamics of Acinetobacter sp. cells in soil were found, and high concentrations of kanamycin reduced the CFU of Acinetobacter sp. cells from 10(9) CFU/g of soil to below detection. In contrast to a chromosomal nptII-encoded kanamycin resistance, the pFG4-generated resistance was found to be unstable over a 31-day incubation period in vitro.
摘要
在此我们表明，基于同源重组，从转基因甜菜中提取的DNA向细菌的水平转移可在土壤中发生。在无菌土壤微宇宙中培养的不动杆菌属菌株BD413(pFG4)细胞中，添加营养物质和编码赋予细菌卡那霉素抗性的功能性nptII基因的转基因植物DNA后，检测到缺失317 bp的nptII基因得以恢复。发现添加卡那霉素对土壤中不动杆菌属细胞群体动态有选择作用，高浓度卡那霉素使不动杆菌属细胞的菌落形成单位从每克土壤10⁹ CFU降至检测限以下。与染色体编码的nptII卡那霉素抗性不同，发现pFG4产生的抗性在体外31天的培养期内不稳定。
相似文献
1
Transformation of Acinetobacter sp. strain BD413(pFG4DeltanptII) with transgenic plant DNA in soil microcosms and effects of kanamycin on selection of transformants.土壤微宇宙中不动杆菌属BD413菌株（pFG4DeltanptII）用转基因植物DNA进行转化及卡那霉素对转化体选择的影响
Appl Environ Microbiol. 2000 Mar;66(3):1237-42. doi: 10.1128/AEM.66.3.1237-1242.2000.
2
Transformation of Acinetobacter sp. strain BD413 by transgenic sugar beet DNA.转基因甜菜DNA对不动杆菌属BD413菌株的转化
Appl Environ Microbiol. 1998 Apr;64(4):1550-4. doi: 10.1128/AEM.64.4.1550-1554.1998.
3
The natural transformation of the soil bacteria Pseudomonas stutzeri and Acinetobacter sp. by transgenic plant DNA strictly depends on homologous sequences in the recipient cells.转基因植物DNA对土壤细菌斯氏假单胞菌和不动杆菌属的自然转化严格依赖于受体细胞中的同源序列。
FEMS Microbiol Lett. 2001 Feb 20;195(2):211-5. doi: 10.1111/j.1574-6968.2001.tb10523.x.
4
Natural transformation of Acinetobacter sp. strain BD413 with cell lysates of Acinetobacter sp., Pseudomonas fluorescens, and Burkholderia cepacia in soil microcosms.在土壤微观环境中，不动杆菌属BD413菌株被不动杆菌属、荧光假单胞菌和洋葱伯克霍尔德氏菌的细胞裂解物自然转化。
Appl Environ Microbiol. 2000 Jan;66(1):206-12. doi: 10.1128/AEM.66.1.206-212.2000.
5
Detection of nptII (kanamycin resistance) genes in genomes of transgenic plants by marker-rescue transformation.通过标记拯救转化检测转基因植物基因组中的nptII（卡那霉素抗性）基因。
Mol Gen Genet. 1998 Apr;257(6):606-13. doi: 10.1007/s004380050688.
6
Transformation of Acinetobacter sp. BD413 with DNA from commercially available genetically modified potato and papaya.用市售转基因马铃薯和木瓜的DNA对不动杆菌属BD413进行转化。
Lett Appl Microbiol. 2006 Aug;43(2):215-21. doi: 10.1111/j.1472-765X.2006.01924.x.
7
Spread of recombinant DNA by roots and pollen of transgenic potato plants, identified by highly specific biomonitoring using natural transformation of an Acinetobacter sp.通过利用不动杆菌属细菌的自然转化进行高度特异性生物监测鉴定转基因马铃薯植株的根和花粉对重组DNA的传播
Appl Environ Microbiol. 2003 Aug;69(8):4455-62. doi: 10.1128/AEM.69.8.4455-4462.2003.
8
Transformation of Acinetobacter baylyi in non-sterile soil using recombinant plant nuclear DNA.利用重组植物核DNA在非无菌土壤中对拜氏不动杆菌进行转化。
Environ Biosafety Res. 2007 Jan-Jun;6(1-2):101-12. doi: 10.1051/ebr:2007024. Epub 2007 Sep 20.
9
Natural transformation and availability of transforming DNA to Acinetobacter calcoaceticus in soil microcosms.土壤微观环境中乙酸钙不动杆菌的自然转化及转化DNA的可利用性
Appl Environ Microbiol. 1997 May;63(5):1945-52. doi: 10.1128/aem.63.5.1945-1952.1997.
10
Long-term persistence and bacterial transformation potential of transplastomic plant DNA in soil.转叶绿体植物 DNA 在土壤中的长期持久性和细菌转化潜能。
Res Microbiol. 2010 Jun;161(5):326-34. doi: 10.1016/j.resmic.2010.04.009. Epub 2010 May 20.
引用本文的文献
1
Dynamics, gene transfer, and ecological function of intracellular and extracellular DNA in environmental microbiome.环境微生物群落中细胞内和细胞外DNA的动力学、基因转移及生态功能
Imeta. 2022 Jun 20;1(3):e34. doi: 10.1002/imt2.34. eCollection 2022 Sep.
2
Plasmid size determines adsorption to clay and breakthrough in a saturated sand column.质粒大小决定其在饱和砂柱中对黏土的吸附及穿透情况。
Heliyon. 2024 Apr 22;10(9):e29679. doi: 10.1016/j.heliyon.2024.e29679. eCollection 2024 May 15.
3
Symbiotic nitrogen fixation and endophytic bacterial community structure in Bt-transgenic chickpea (Cicer arietinum L).转 Bt 基因鹰嘴豆（Cicer arietinum L.）中的共生固氮作用和内生细菌群落结构
Sci Rep. 2020 Mar 25;10(1):5453. doi: 10.1038/s41598-020-62199-1.
4
Detecting rare gene transfer events in bacterial populations.检测细菌群体中的罕见基因转移事件。
Front Microbiol. 2014 Jan 7;4:415. doi: 10.3389/fmicb.2013.00415.
5
An Arabidopsis thaliana ABC transporter that confers kanamycin resistance in transgenic plants does not endow resistance to Escherichia coli.拟南芥 ABC 转运蛋白可使转基因植物获得卡那霉素抗性，但不能赋予大肠杆菌抗性。
Microb Biotechnol. 2008 Mar;1(2):191-5. doi: 10.1111/j.1751-7915.2007.00010.x.
6
Development of real time PCR assays for detection and quantification of transgene DNA of a Bacillus thuringiensis (Bt) corn hybrid in soil samples.实时 PCR 检测方法的建立与优化，用于定量检测土壤样品中苏云金芽孢杆菌（Bt）玉米杂交种的转基因 DNA。
Transgenic Res. 2010 Oct;19(5):765-74. doi: 10.1007/s11248-009-9353-1. Epub 2010 Jan 1.
7
Sexual isolation in Acinetobacter baylyi is locus-specific and varies 10,000-fold over the genome.贝氏不动杆菌的性隔离是特定基因座的，在基因组上变化 10000 倍。
Genetics. 2009 Aug;182(4):1165-81. doi: 10.1534/genetics.109.103127. Epub 2009 May 27.
8
Impact of cry1AC-carrying Brassica rapa subsp. pekinensis on leaf bacterial community.携带cry1AC的白菜型油菜（芜菁亚种）对叶片细菌群落的影响。
J Microbiol. 2009 Feb;47(1):33-9. doi: 10.1007/s12275-008-0254-4. Epub 2009 Feb 20.
9
Will transgenic plants adversely affect the environment?转基因植物会对环境产生不利影响吗？
J Biosci. 2005 Sep;30(4):515-48. doi: 10.1007/BF02703726.
10
Unsuccessful search for DNA transfer from transgenic plants to bacteria in the intestine of the tobacco horn worm, Manduca sexta.
Transgenic Res. 2005 Apr;14(2):207-15. doi: 10.1007/s11248-004-6701-z.
本文引用的文献
1
Random amplified polymorphic DNA and pedigree relationships in spring barley.随机扩增多态性 DNA 与春大麦的系谱关系。
Theor Appl Genet. 1993 Feb;85(8):976-84. doi: 10.1007/BF00215037.
2
Induced Natural Transformation of Acinetobacter calcoaceticus in Soil Microcosms.土壤微宇宙中不动杆菌的诱导自然转化。
Appl Environ Microbiol. 1997 Oct;63(10):3972-7. doi: 10.1128/aem.63.10.3972-3977.1997.
3
Production of the antibiotic phenazine-1-carboxylic Acid by fluorescent pseudomonas species in the rhizosphere of wheat.荧光假单胞菌在小麦根际产生抗生素吩嗪-1-羧酸。
Appl Environ Microbiol. 1990 Apr;56(4):908-12. doi: 10.1128/aem.56.4.908-912.1990.
4
Natural transformation of Acinetobacter sp. strain BD413 with cell lysates of Acinetobacter sp., Pseudomonas fluorescens, and Burkholderia cepacia in soil microcosms.在土壤微观环境中，不动杆菌属BD413菌株被不动杆菌属、荧光假单胞菌和洋葱伯克霍尔德氏菌的细胞裂解物自然转化。
Appl Environ Microbiol. 2000 Jan;66(1):206-12. doi: 10.1128/AEM.66.1.206-212.2000.
5
Rickettsiae and Chlamydiae: evidence of horizontal gene transfer and gene exchange.立克次氏体和衣原体：水平基因转移与基因交换的证据
Trends Genet. 1999 May;15(5):173-5. doi: 10.1016/s0168-9525(99)01704-7.
6
Horizontal gene transfer from transgenic plants to terrestrial bacteria--a rare event?从转基因植物到陆生细菌的水平基因转移——是罕见事件吗？
FEMS Microbiol Rev. 1998 Jun;22(2):79-103. doi: 10.1111/j.1574-6976.1998.tb00362.x.
7
Molecular archaeology of the Escherichia coli genome.大肠杆菌基因组的分子考古学
Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9413-7. doi: 10.1073/pnas.95.16.9413.
8
"Horizontal" gene transfer from a transgenic potato line to a bacterial pathogen (Erwinia chrysanthemi) occurs--if at all--at an extremely low frequency.从转基因马铃薯品系到细菌病原体（菊欧文氏菌）的“水平”基因转移即使真的发生，频率也极低。
Biotechnology (N Y). 1995 Oct;13(10):1094-8. doi: 10.1038/nbt1095-1094.
9
Detection of nptII (kanamycin resistance) genes in genomes of transgenic plants by marker-rescue transformation.通过标记拯救转化检测转基因植物基因组中的nptII（卡那霉素抗性）基因。
Mol Gen Genet. 1998 Apr;257(6):606-13. doi: 10.1007/s004380050688.
10
Transformation of Acinetobacter sp. strain BD413 by transgenic sugar beet DNA.转基因甜菜DNA对不动杆菌属BD413菌株的转化
Appl Environ Microbiol. 1998 Apr;64(4):1550-4. doi: 10.1128/AEM.64.4.1550-1554.1998.
Zotero 插件