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Transformation of Acinetobacter sp. strain BD413(pFG4DeltanptII) with transgenic plant DNA in soil microcosms and effects of kanamycin on selection of transformants.土壤微宇宙中不动杆菌属BD413菌株（pFG4DeltanptII）用转基因植物DNA进行转化及卡那霉素对转化体选择的影响
Appl Environ Microbiol. 2000 Mar;66(3):1237-42. doi: 10.1128/AEM.66.3.1237-1242.2000.
2
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3
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本文引用的文献

1
Random amplified polymorphic DNA and pedigree relationships in spring barley.随机扩增多态性 DNA 与春大麦的系谱关系。
Theor Appl Genet. 1993 Feb;85(8):976-84. doi: 10.1007/BF00215037.
2
Induced Natural Transformation of Acinetobacter calcoaceticus in Soil Microcosms.土壤微宇宙中不动杆菌的诱导自然转化。
Appl Environ Microbiol. 1997 Oct;63(10):3972-7. doi: 10.1128/aem.63.10.3972-3977.1997.
3
Production of the antibiotic phenazine-1-carboxylic Acid by fluorescent pseudomonas species in the rhizosphere of wheat.荧光假单胞菌在小麦根际产生抗生素吩嗪-1-羧酸。
Appl Environ Microbiol. 1990 Apr;56(4):908-12. doi: 10.1128/aem.56.4.908-912.1990.
4
Natural transformation of Acinetobacter sp. strain BD413 with cell lysates of Acinetobacter sp., Pseudomonas fluorescens, and Burkholderia cepacia in soil microcosms.在土壤微观环境中，不动杆菌属BD413菌株被不动杆菌属、荧光假单胞菌和洋葱伯克霍尔德氏菌的细胞裂解物自然转化。
Appl Environ Microbiol. 2000 Jan;66(1):206-12. doi: 10.1128/AEM.66.1.206-212.2000.
5
Rickettsiae and Chlamydiae: evidence of horizontal gene transfer and gene exchange.立克次氏体和衣原体：水平基因转移与基因交换的证据
Trends Genet. 1999 May;15(5):173-5. doi: 10.1016/s0168-9525(99)01704-7.
6
Horizontal gene transfer from transgenic plants to terrestrial bacteria--a rare event?从转基因植物到陆生细菌的水平基因转移——是罕见事件吗？
FEMS Microbiol Rev. 1998 Jun;22(2):79-103. doi: 10.1111/j.1574-6976.1998.tb00362.x.
7
Molecular archaeology of the Escherichia coli genome.大肠杆菌基因组的分子考古学
Proc Natl Acad Sci U S A. 1998 Aug 4;95(16):9413-7. doi: 10.1073/pnas.95.16.9413.
8
"Horizontal" gene transfer from a transgenic potato line to a bacterial pathogen (Erwinia chrysanthemi) occurs--if at all--at an extremely low frequency.从转基因马铃薯品系到细菌病原体（菊欧文氏菌）的“水平”基因转移即使真的发生，频率也极低。
Biotechnology (N Y). 1995 Oct;13(10):1094-8. doi: 10.1038/nbt1095-1094.
9
Detection of nptII (kanamycin resistance) genes in genomes of transgenic plants by marker-rescue transformation.通过标记拯救转化检测转基因植物基因组中的nptII（卡那霉素抗性）基因。
Mol Gen Genet. 1998 Apr;257(6):606-13. doi: 10.1007/s004380050688.
10
Transformation of Acinetobacter sp. strain BD413 by transgenic sugar beet DNA.转基因甜菜DNA对不动杆菌属BD413菌株的转化
Appl Environ Microbiol. 1998 Apr;64(4):1550-4. doi: 10.1128/AEM.64.4.1550-1554.1998.

土壤微宇宙中不动杆菌属BD413菌株（pFG4DeltanptII）用转基因植物DNA进行转化及卡那霉素对转化体选择的影响

Transformation of Acinetobacter sp. strain BD413(pFG4DeltanptII) with transgenic plant DNA in soil microcosms and effects of kanamycin on selection of transformants.

作者信息

Nielsen K M, van Elsas J D, Smalla K

机构信息

Unigen and Department of Botany, Norwegian University of Science and Technology, 7491 Trondheim, Norway.

出版信息

Appl Environ Microbiol. 2000 Mar;66(3):1237-42. doi: 10.1128/AEM.66.3.1237-1242.2000.

DOI:10.1128/AEM.66.3.1237-1242.2000
PMID:10698801
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC91972/
Abstract

Here we show that horizontal transfer of DNA, extracted from transgenic sugar beets, to bacteria, based on homologous recombination, can occur in soil. Restoration of a 317-bp-deleted nptII gene in Acinetobacter sp. strain BD413(pFG4) cells incubated in sterile soil microcosms was detected after addition of nutrients and transgenic plant DNA encoding a functional nptII gene conferring bacterial kanamycin resistance. Selective effects of the addition of kanamycin on the population dynamics of Acinetobacter sp. cells in soil were found, and high concentrations of kanamycin reduced the CFU of Acinetobacter sp. cells from 10(9) CFU/g of soil to below detection. In contrast to a chromosomal nptII-encoded kanamycin resistance, the pFG4-generated resistance was found to be unstable over a 31-day incubation period in vitro.

摘要

在此我们表明，基于同源重组，从转基因甜菜中提取的DNA向细菌的水平转移可在土壤中发生。在无菌土壤微宇宙中培养的不动杆菌属菌株BD413(pFG4)细胞中，添加营养物质和编码赋予细菌卡那霉素抗性的功能性nptII基因的转基因植物DNA后，检测到缺失317 bp的nptII基因得以恢复。发现添加卡那霉素对土壤中不动杆菌属细胞群体动态有选择作用，高浓度卡那霉素使不动杆菌属细胞的菌落形成单位从每克土壤10⁹ CFU降至检测限以下。与染色体编码的nptII卡那霉素抗性不同，发现pFG4产生的抗性在体外31天的培养期内不稳定。