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2
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转基因甜菜DNA对不动杆菌属BD413菌株的转化

Transformation of Acinetobacter sp. strain BD413 by transgenic sugar beet DNA.

作者信息

Gebhard F, Smalla K

机构信息

Biologische Bundesanstalt für Land- und Forstwirtschaft, Institut für Biochemie und Pflanzenvirologie, Braunschweig, Germany.

出版信息

Appl Environ Microbiol. 1998 Apr;64(4):1550-4. doi: 10.1128/AEM.64.4.1550-1554.1998.

DOI:10.1128/AEM.64.4.1550-1554.1998
PMID:9546192
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC106187/
Abstract

The ability of Acinetobacter sp. strain BD413(pFG4 delta nptII) to take up and integrate transgenic plant DNA based on homologous recombination was studied under optimized laboratory conditions. Restoration of nptII, resulting in kanamycin-resistant transformants, was observed with plasmid DNA, plant DNA, and homogenates carrying the gene nptII. Molecular analysis showed that some transformants not only restored the 317-bp deletion but also obtained additional DNA.

摘要

在优化的实验室条件下，研究了不动杆菌属菌株BD413(pFG4 delta nptII)基于同源重组摄取和整合转基因植物DNA的能力。用携带nptII基因的质粒DNA、植物DNA和匀浆观察到nptII的恢复，产生了卡那霉素抗性转化体。分子分析表明，一些转化体不仅恢复了317bp的缺失，还获得了额外的DNA。