Segonds C, Bingen E, Couetdic G, Mathy S, Brahimi N, Marty N, Plesiat P, Michel-Briand Y, Chabanon G
Laboratoire de Bactériologie-Virologie-Hygiène, CHU Rangueil, Toulouse, France.
J Clin Microbiol. 1997 Aug;35(8):2055-60. doi: 10.1128/jcm.35.8.2055-2060.1997.
Burkholderia cepacia has been involved in outbreaks of pulmonary infection among patients with cystic fibrosis (CF), and the spread of a highly transmissible clone has been reported throughout the United Kingdom and Canada. These data prompted a DNA-based typing study of the strains recovered in French CF centers. Ninety-five isolates recovered from 71 patients attending 13 CF centers in 9 regions of France were characterized by randomly amplified polymorphic DNA (RAPD) analysis and pulsed-field gel electrophoresis (PFGE). Twenty-one genotypes were identified among the 95 isolates, and the results of RAPD and PFGE were concordant for 89 isolates (94%). Cross-colonization was demonstrated in 7 of the 13 CF centers. The investigation of serial isolates showed that most chronically colonized patients harbored a single B. cepacia strain. A geographically clustered distribution of B. cepacia genotypes was observed, except for one genotype, which was detected in four regions but was proven to be different from the genotype of the British-Canadian highly transmissible strain. The present study confirms the ability of B. cepacia to spread among CF communities in France and the importance of epidemiological surveys in the institution of prevention policies.
洋葱伯克霍尔德菌已引发囊性纤维化（CF）患者的肺部感染暴发，且据报道一种高传播性克隆株已在英国和加拿大传播。这些数据促使对法国CF中心分离出的菌株进行基于DNA的分型研究。从法国9个地区13个CF中心的71名患者中分离出95株菌株，通过随机扩增多态性DNA（RAPD）分析和脉冲场凝胶电泳（PFGE）对其进行特征分析。在95株分离株中鉴定出21种基因型，RAPD和PFGE结果在89株分离株（94%）中一致。在13个CF中心中的7个中心发现了交叉定植情况。对连续分离株的调查表明，大多数长期定植患者携带单一的洋葱伯克霍尔德菌菌株。观察到洋葱伯克霍尔德菌基因型存在地理聚集分布，但有一种基因型在四个地区被检测到，且被证明与英国 - 加拿大高传播性菌株的基因型不同。本研究证实了洋葱伯克霍尔德菌在法国CF群体中传播的能力以及流行病学调查在制定预防政策中的重要性。
