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转化后细胞糖蛋白的变化：在十二烷基硫酸钠凝胶中鉴定特定糖蛋白和抗原

Changes in cellular glycoproteins after transformation: identification of specific glycoproteins and antigens in sodium dodecyl sulfate gels.

作者信息

Burridge K

出版信息

Proc Natl Acad Sci U S A. 1976 Dec;73(12):4457-61. doi: 10.1073/pnas.73.12.4457.

DOI:10.1073/pnas.73.12.4457
PMID:1069997
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC431497/
Abstract

The glycoproteins of whole cells have been analyzed by direct application of radio-iodinated lectins to sodium dodecyl sulfate gels, followed by autoradiography. By use of lectins with different carbohydrate specificities, different sets of glycoproteins have been visualized. The most prominent lectin-binding band in many gels is thelarge, external, transformation-sensitive (LETS) protein. Major glycoprotein differences are revealed when normal and virus-transformed cells are compared. Certain differences, however, are also seen when the glycoproteins are compared from two separately derived simian virus 40 transformants of 3T3 cells, suggesting a degree of clonal variation between these lines that may not relate to transformation. A complementary technique is used to detect specific antigens in sodium dodecyl sulfate gels by direct application of iodinated antibodies. An antiserum specific for the LETS protein is used to identify this antigen in the gels of both normal and transformed cells.

摘要

通过将放射性碘化凝集素直接应用于十二烷基硫酸钠凝胶，随后进行放射自显影，对全细胞的糖蛋白进行了分析。使用具有不同碳水化合物特异性的凝集素，可以观察到不同组的糖蛋白。许多凝胶中最突出的凝集素结合带是大的、细胞外的、转化敏感（LETS）蛋白。比较正常细胞和病毒转化细胞时，会发现主要的糖蛋白差异。然而，当比较来自3T3细胞的两个分别衍生的猿猴病毒40转化体的糖蛋白时，也会看到某些差异，这表明这些细胞系之间存在一定程度的克隆变异，可能与转化无关。一种互补技术用于通过直接应用碘化抗体在十二烷基硫酸钠凝胶中检测特定抗原。使用对LETS蛋白特异的抗血清在正常细胞和转化细胞的凝胶中鉴定该抗原。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4274/431497/71cad404e236/pnas00042-0214-c.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4274/431497/71cad404e236/pnas00042-0214-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4274/431497/c373ae837261/pnas00042-0212-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4274/431497/f22b88a02efd/pnas00042-0213-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4274/431497/60d8d06efb1e/pnas00042-0213-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4274/431497/365d6e32d0d6/pnas00042-0213-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4274/431497/7663be1fe01a/pnas00042-0213-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4274/431497/21a1c6dc62d6/pnas00042-0214-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4274/431497/2bfa4d00901c/pnas00042-0214-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4274/431497/71cad404e236/pnas00042-0214-c.jpg

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