Bouamrane F, Assidjo N E, Bouteille B, Dreyfuss M F, Dardé M L, Cardot P J
Laboratoire de Chimie Analytique et de Bromatologie, Université de Limoges, Faculté de Pharmacie, France.
J Pharm Biomed Anal. 1999 Jul;20(3):503-12. doi: 10.1016/s0731-7085(99)00049-7.
Toxoplasmosis is a worldwide disease caused by Toxoplasma gondii, an intracellular protozoa of micronic size range (4-10 microm). Its classical purification processes are complex and often associated with low recovery. All investigation procedures concerning this parasite require its isolation and purification from at least the mouse ascitic fluid. For this purpose, a recently developed laboratory technology was used, i.e. sedimentation field-flow fractionation. This chromatographic-like separation technology was demonstrated to be particularly selective for isolation and separation of micron-sized biological particles. Sedimentation field-flow fractionation operated on the steric-hyperlayer mode was used to isolate the parasite from the remanent ascitic contaminants of different origins and from red blood cells. With this technology, 86% recovery with 97% viability was obtained in less than 30 min.
弓形虫病是一种由刚地弓形虫引起的全球性疾病，刚地弓形虫是一种微小尺寸范围（4 - 10微米）的细胞内原生动物。其经典的纯化过程复杂，且回收率往往较低。所有关于这种寄生虫的研究程序都需要从至少小鼠腹水中分离和纯化它。为此，使用了一种最近开发的实验室技术，即沉降场流分级分离法。这种类似色谱的分离技术被证明对微米级生物颗粒的分离和纯化具有特别的选择性。以空间超层模式运行的沉降场流分级分离法用于从不同来源的残余腹水污染物和红细胞中分离寄生虫。使用这项技术，在不到30分钟的时间内获得了86%的回收率和97%的活力。
