流动注射化学发光法测定药物制剂及人血清中戊酸雌二醇的含量
Determination of estradiol valerate in pharmaceutical preparations and human serum by flow injection chemiluminescence.
机构信息
Key Laboratory of Chemical Sensing & Analysis in Universities of Shandong (University of Jinan), School of Chemistry and Chemical Engineering, University of Jinan, Jinan, 250022, China.
出版信息
Luminescence. 2013 May-Jun;28(3):407-11. doi: 10.1002/bio.2397. Epub 2012 Jul 9.
A novel method for the detection of trace estradiol valerate (EV) in pharmaceutical preparations and human serum was developed by inhibition of luminol chemiluminescence (CL) by estradiol valerate on the zinc deuteroporphyrin (ZnDP)-enhanced luminol-K3 Fe(CN)6 chemiluminescence system. Under optimized experimental conditions, CL intensity and concentration of estradiol valerate had a good linear relationship in the ranges of 8.0 × 10(-8) to 1.0 × 10(-5) g/mL. Detection limit (3σ) was estimated to be 3.5 × 10(-8) g/mL. The proposed method was applied successfully for the determination of estradiol valerate in pharmaceutical preparations and human serum and recoveries were 97.0-105.0% and 95.5-106.0%, respectively. The possible mechanism of the CL system is discussed.
建立了一种新的方法，用于检测药物制剂和人血清中的痕量戊酸雌二醇（EV）。该方法基于戊酸雌二醇对锌原卟啉（ZnDP）增强鲁米诺-K3 Fe（CN）6 化学发光体系中鲁米诺化学发光（CL）的抑制作用。在优化的实验条件下，CL 强度与戊酸雌二醇的浓度在 8.0×10(-8)到 1.0×10(-5) g/mL 范围内呈良好的线性关系。检测限（3σ）估计为 3.5×10(-8) g/mL。该方法成功地应用于药物制剂和人血清中戊酸雌二醇的测定，回收率分别为 97.0-105.0%和 95.5-106.0%。讨论了 CL 体系的可能机制。
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