枯草芽孢杆菌中的限制与修饰：温和噬菌体phi 3T中编码BsuR特异性修饰甲基转移酶的基因。
Restriction and modification in Bacillus subtilis: gene coding for a BsuR-specific modification methyltransferase in the temperate bacteriophage phi 3T.
作者信息
Noyer-Weidner M, Pawlek B, Jentsch S, Günthert U, Trautner T A
出版信息
J Virol. 1981 Jun;38(3):1077-80. doi: 10.1128/JVI.38.3.1077-1080.1981.
Abstract
The resistance of Phi3T DNA to degradation by the restriction enzyme BsuR or its isoschizomer HaeIII is due to obligatory modification of such DNA. Biochemical and genetical experiments indicate that Phi3T codes for a methyltransferase, which methylates Phi3T DNA itself or heterologous DNA at target sites 5'-GG(*)CC.
摘要
Phi3T DNA 对限制性内切酶 BsuR 或其同裂酶 HaeIII 的降解具有抗性，这归因于此类 DNA 的必然修饰。生化和遗传学实验表明，Phi3T 编码一种甲基转移酶，该酶可在靶位点 5'-GG(*)CC 处使 Phi3T DNA 自身或异源 DNA 发生甲基化。
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