Couwenhoven R, Schwartz S A
Department of Pathology, University of Health Sciences, Chicago Medical School, IL 60064.
Arch Oral Biol. 1988;33(1):57-63. doi: 10.1016/0003-9969(88)90096-9.
The enamel organ of the mammalian dental primordium undergoes a precise sequence of differentiation. To correlate this differentiation with tissue-specific markers we analysed the keratin protein composition and immunoreactivity of incisor primordia from the earliest stage of odontogenesis to the neonatal period. Throughout the enamel organ synthesized a characteristic subset of keratin proteins, and the expression of one specific keratin marked the onset of the cap stage. Interestingly, the immunoreactivity of the ameloblastic keratins against polyclonal antibodies increased with progressive odontogenesis, suggesting that cytokeratin filaments may undergo post-translational or conformational alterations during assembly within differentiating enamel-organ cells.
哺乳动物牙原基的成釉器经历精确的分化序列。为了将这种分化与组织特异性标志物相关联，我们分析了从牙发生最早阶段到新生儿期的切牙原基的角蛋白蛋白质组成和免疫反应性。在整个成釉器中合成了一组特征性的角蛋白，一种特定角蛋白的表达标志着帽状期的开始。有趣的是，成釉细胞角蛋白针对多克隆抗体的免疫反应性随着牙发生的进展而增加，这表明细胞角蛋白丝在分化的成釉器细胞内组装过程中可能经历翻译后或构象改变。
